Detailed information of TA system TA029585

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5306979..5307574
• Replicon: chromosome
• Accession: NC_021577
• Organism: Pseudomonas aeruginosa RP73

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A241XLJ5
• Locus tag: M062_RS30210
• Protein ID: WP_003117425.1
• Coordinates: 5307296..5307574 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: M062_RS24575
• Protein ID: WP_003113527.1
• Coordinates: 5306979..5307284 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M062_RS24540	5302118..5302966	+	849	WP_003146058.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
M062_RS24550	5303133..5304074	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
M062_RS24555	5304191..5304805	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
M062_RS24560	5304847..5305431	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
M062_RS24565	5305472..5306572	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
M062_RS24575	5306979..5307284	-	306	WP_003113527.1	HigA family addiction module antidote protein	Antitoxin
M062_RS30210	5307296..5307574	-	279	WP_003117425.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M062_RS24580	5307903..5310131	+	2229	WP_003113525.1	TonB-dependent receptor	-
M062_RS24585	5310201..5310848	-	648	WP_003095021.1	carbonate dehydratase	-
M062_RS24590	5310910..5312148	-	1239	WP_014603324.1	C69 family dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-69)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10701.28 Da
• Isoelectric Point: 8.5576

>T29585 WP_003117425.1 NC_021577:c5307574-5307296 [Pseudomonas aeruginosa RP73]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAARELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T29585 NC_021577:c5307574-5307296 [Pseudomonas aeruginosa RP73]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTAGGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11247.81 Da
• Isoelectric Point: 5.0747

>AT29585 WP_003113527.1 NC_021577:c5307284-5306979 [Pseudomonas aeruginosa RP73]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT29585 NC_021577:c5307284-5306979 [Pseudomonas aeruginosa RP73]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTTGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: A0A241XLJ5