Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3572264..3572958 Replicon chromosome
Accession NZ_OW967795
Organism Escherichia coli isolate 538

Toxin (Protein)


Gene name yafO Uniprot ID S1PJQ2
Locus tag LQ148_RS17330 Protein ID WP_001263500.1
Coordinates 3572264..3572662 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag LQ148_RS17335 Protein ID WP_000554758.1
Coordinates 3572665..3572958 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ148_RS17305 (3567630) 3567630..3568088 - 459 WP_001291991.1 xanthine phosphoribosyltransferase -
LQ148_RS17310 (3568349) 3568349..3569806 + 1458 WP_001293022.1 cytosol nonspecific dipeptidase -
LQ148_RS17315 (3569862) 3569862..3570476 - 615 WP_000602123.1 peptide chain release factor H -
LQ148_RS17320 (3570473) 3570473..3571612 - 1140 WP_022296578.1 RNA ligase RtcB family protein -
LQ148_RS17325 (3571802) 3571802..3572254 - 453 WP_071589393.1 GNAT family N-acetyltransferase -
LQ148_RS17330 (3572264) 3572264..3572662 - 399 WP_001263500.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
LQ148_RS17335 (3572665) 3572665..3572958 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
LQ148_RS17340 (3573010) 3573010..3574065 - 1056 WP_001226168.1 DNA polymerase IV -
LQ148_RS17345 (3574136) 3574136..3574921 - 786 WP_000207551.1 putative lateral flagellar export/assembly protein LafU -
LQ148_RS17350 (3574893) 3574893..3576605 + 1713 Protein_3400 flagellar biosynthesis protein FlhA -
LQ148_RS17355 (3576721) 3576721..3577218 - 498 WP_000006239.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - gtrB 3519720..3578143 58423


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15413.85 Da        Isoelectric Point: 8.9511

>T295839 WP_001263500.1 NZ_OW967795:c3572662-3572264 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT295839 WP_000554758.1 NZ_OW967795:c3572958-3572665 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PJQ2

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References