Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3312800..3313479 Replicon chromosome
Accession NZ_OW967795
Organism Escherichia coli isolate 538

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag LQ148_RS16030 Protein ID WP_000057523.1
Coordinates 3313177..3313479 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag LQ148_RS16025 Protein ID WP_000806442.1
Coordinates 3312800..3313141 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ148_RS16015 (3309044) 3309044..3309976 - 933 WP_000883041.1 glutaminase A -
LQ148_RS16020 (3310238) 3310238..3312742 + 2505 WP_225387190.1 copper-exporting P-type ATPase CopA -
LQ148_RS16025 (3312800) 3312800..3313141 - 342 WP_000806442.1 HigA family addiction module antitoxin Antitoxin
LQ148_RS16030 (3313177) 3313177..3313479 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LQ148_RS16035 (3313612) 3313612..3314406 + 795 WP_022296631.1 TraB/GumN family protein -
LQ148_RS16040 (3314610) 3314610..3315089 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
LQ148_RS16045 (3315126) 3315126..3316778 - 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
LQ148_RS16050 (3316996) 3316996..3318216 + 1221 WP_022296630.1 fosmidomycin MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T295837 WP_000057523.1 NZ_OW967795:c3313479-3313177 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT295837 WP_000806442.1 NZ_OW967795:c3313141-3312800 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References