Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1175580..1176307 Replicon chromosome
Accession NZ_OW967388
Organism Enterobacter cloacae isolate 114

Toxin (Protein)


Gene name higB Uniprot ID A0A8B3V067
Locus tag LQ218_RS05550 Protein ID WP_022650547.1
Coordinates 1175996..1176307 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B3V464
Locus tag LQ218_RS05545 Protein ID WP_022650546.1
Coordinates 1175580..1175999 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ218_RS05530 (AI2624V1_1074) 1172652..1173773 - 1122 WP_003858868.1 YbdK family carboxylate-amine ligase -
LQ218_RS05535 (AI2624V1_1075) 1173879..1174769 + 891 WP_022650544.1 oxidoreductase -
LQ218_RS05540 (AI2624V1_1076) 1174822..1175256 - 435 WP_022650545.1 hypothetical protein -
LQ218_RS05545 (AI2624V1_1077) 1175580..1175999 - 420 WP_022650546.1 helix-turn-helix domain-containing protein Antitoxin
LQ218_RS05550 1175996..1176307 - 312 WP_022650547.1 type II toxin-antitoxin system HigB family toxin Toxin
LQ218_RS05555 (AI2624V1_1078) 1176763..1177419 + 657 WP_022650548.1 hypothetical protein -
LQ218_RS05560 (AI2624V1_1079) 1177532..1177987 - 456 WP_022650549.1 hypothetical protein -
LQ218_RS05565 (AI2624V1_1080) 1178270..1179151 - 882 WP_022650550.1 LysR family transcriptional regulator -
LQ218_RS05570 (AI2624V1_1081) 1179257..1180036 + 780 WP_003858848.1 acetolactate decarboxylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(83-134)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12554.34 Da        Isoelectric Point: 9.9435

>T295803 WP_022650547.1 NZ_OW967388:c1176307-1175996 [Enterobacter cloacae]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKF
FIKHISTHAEYDKLTDYYRRTKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15447.51 Da        Isoelectric Point: 4.6051

>AT295803 WP_022650546.1 NZ_OW967388:c1175999-1175580 [Enterobacter cloacae]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVEMLTAKIDKYENESPEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B3V067

Antitoxin

Source ID Structure
AlphaFold DB A0A8B3V464

References