Detailed information of TA system TA029580

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 861926..862607
• Replicon: chromosome
• Accession: NC_021577
• Organism: Pseudomonas aeruginosa RP73

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: M062_RS04020
• Protein ID: WP_015503432.1
• Coordinates: 861926..862291 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: M062_RS04025
• Protein ID: WP_016561576.1
• Coordinates: 862284..862607 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M062_RS03990	857931..858365	-	435	WP_016253806.1	RidA family protein	-
M062_RS03995	858586..859539	+	954	WP_003085661.1	LysR family transcriptional regulator	-
M062_RS04000	859512..860396	-	885	WP_003454589.1	LysR family transcriptional regulator	-
M062_RS04005	860497..860922	+	426	WP_003114206.1	ring-cleaving dioxygenase	-
M062_RS04010	860941..861213	-	273	WP_003085667.1	hypothetical protein	-
M062_RS04015	861420..861662	+	243	WP_043884955.1	hypothetical protein	-
M062_RS04020	861926..862291	+	366	WP_015503432.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M062_RS04025	862284..862607	+	324	WP_016561576.1	XRE family transcriptional regulator	Antitoxin
M062_RS30380	862723..862842	-	120	Protein_797	IS5/IS1182 family transposase	-
M062_RS04035	862897..863229	+	333	WP_016561578.1	hypothetical protein	-
M062_RS29455	863229..863504	+	276	WP_004353146.1	hypothetical protein	-
M062_RS29460	863679..864221	+	543	WP_019396018.1	tyrosine-type recombinase/integrase	-
M062_RS29465	864236..864463	-	228	WP_014603215.1	hypothetical protein	-
M062_RS04045	865066..865944	-	879	WP_003109435.1	hypothetical protein	-
M062_RS04050	866032..866715	+	684	WP_003085795.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-63)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13798.14 Da
• Isoelectric Point: 4.8219

>T29580 WP_015503432.1 NC_021577:861926-862291 [Pseudomonas aeruginosa RP73]
VAWDIEYTDEFGDWWGSLSEDEQESLAVTVRLLEERGPSLGHPHSSGINGSRPGHMRELRTQHGGRPFRTLYAFDPRRSA
ILPIGGDKTGDDRWYELNVPIADRLHDEHLHQLREEGLIDG

Nucleotide

• Length: 366 bp

>T29580 NC_021577:861926-862291 [Pseudomonas aeruginosa RP73]
GTGGCGTGGGATATCGAGTACACCGACGAGTTCGGTGATTGGTGGGGTTCTCTGTCCGAGGATGAGCAGGAGTCGCTAGC
TGTGACGGTTCGCTTGCTTGAGGAGCGGGGGCCATCCTTGGGGCACCCCCACAGCAGCGGCATCAATGGCTCCCGCCCTG
GCCATATGAGAGAGCTACGGACTCAACACGGCGGGCGACCTTTCCGAACGCTTTACGCGTTTGACCCCAGACGCTCCGCG
ATACTGCCGATCGGTGGCGACAAGACTGGCGATGATCGTTGGTACGAATTGAACGTGCCCATTGCCGACCGCCTCCATGA
CGAACACTTGCACCAGCTCCGTGAGGAGGGACTGATCGATGGCTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11903.72 Da
• Isoelectric Point: 5.8900

>AT29580 WP_016561576.1 NC_021577:862284-862607 [Pseudomonas aeruginosa RP73]
MAKKFSDLRAQMSPEAQARVEAKAQELLAEMPLNELRQSRGLSQKMLSEVLQVQQPAIAKMERRTDMYISTLRSHIEAMG
GQLEVIARFPDGAVKISNFADIEEAGA

Nucleotide

• Length: 324 bp

>AT29580 NC_021577:862284-862607 [Pseudomonas aeruginosa RP73]
ATGGCTAAGAAATTCTCCGACCTTCGGGCGCAAATGTCGCCTGAGGCACAGGCCCGCGTCGAGGCCAAGGCCCAGGAACT
GTTGGCTGAAATGCCCCTGAATGAGTTGCGCCAGTCTAGAGGCTTGTCGCAGAAGATGCTGTCTGAAGTGCTGCAGGTGC
AGCAGCCGGCAATTGCCAAGATGGAGCGGCGTACGGATATGTACATCTCCACCTTGCGCAGCCACATCGAAGCCATGGGC
GGCCAGCTGGAAGTGATTGCACGCTTCCCGGATGGTGCGGTGAAAATCAGCAACTTCGCGGATATCGAAGAGGCGGGCGC
CTAA