Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1116622..1117349 Replicon chromosome
Accession NZ_OW967178
Organism Escherichia coli isolate 362

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag LQ124_RS05280 Protein ID WP_000547555.1
Coordinates 1116622..1116933 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LQ124_RS05285 Protein ID WP_000126294.1
Coordinates 1116930..1117349 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ124_RS05255 (1112557) 1112557..1114266 + 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -
LQ124_RS05260 (1114276) 1114276..1114818 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
LQ124_RS05265 (1114818) 1114818..1115585 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
LQ124_RS05270 (1115582) 1115582..1115992 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
LQ124_RS05275 (1115985) 1115985..1116455 + 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
LQ124_RS05280 (1116622) 1116622..1116933 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
LQ124_RS05285 (1116930) 1116930..1117349 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
LQ124_RS05290 (1117428) 1117428..1118852 - 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
LQ124_RS05295 (1118861) 1118861..1120318 - 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
LQ124_RS05300 (1120578) 1120578..1121588 + 1011 WP_032141326.1 DNA-binding transcriptional regulator AscG -
LQ124_RS05305 (1121737) 1121737..1122264 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T295757 WP_000547555.1 NZ_OW967178:1116622-1116933 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT295757 WP_000126294.1 NZ_OW967178:1116930-1117349 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References