Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2069525..2070219 Replicon chromosome
Accession NZ_OW967003
Organism Escherichia coli isolate 624

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag LQ179_RS10045 Protein ID WP_001263491.1
Coordinates 2069525..2069923 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag LQ179_RS10050 Protein ID WP_000554755.1
Coordinates 2069926..2070219 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ179_RS10015 (2064787) 2064787..2066244 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
LQ179_RS10020 (2066253) 2066253..2066534 + 282 WP_022645226.1 hypothetical protein -
LQ179_RS10025 (2066551) 2066551..2067060 - 510 WP_001361775.1 metal-dependent hydrolase -
LQ179_RS10030 (2067122) 2067122..2067736 - 615 WP_022645225.1 peptide chain release factor H -
LQ179_RS10035 (2067733) 2067733..2068872 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
LQ179_RS10040 (2069063) 2069063..2069515 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
LQ179_RS10045 (2069525) 2069525..2069923 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
LQ179_RS10050 (2069926) 2069926..2070219 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
LQ179_RS10055 (2070271) 2070271..2071326 - 1056 WP_022645222.1 DNA polymerase IV -
LQ179_RS10060 (2071397) 2071397..2072182 - 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
LQ179_RS10065 (2072154) 2072154..2073866 + 1713 Protein_1979 flagellar biosynthesis protein FlhA -
LQ179_RS10070 (2073964) 2073964..2074737 - 774 WP_022645219.1 C40 family peptidase -
LQ179_RS10075 (2074923) 2074923..2075183 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T295740 WP_001263491.1 NZ_OW967003:c2069923-2069525 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT295740 WP_000554755.1 NZ_OW967003:c2070219-2069926 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References