Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3725730..3726271 Replicon chromosome
Accession NZ_OW849377
Organism Escherichia coli isolate 162

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag LQ158_RS18000 Protein ID WP_000615976.1
Coordinates 3725993..3726271 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag LQ158_RS17995 Protein ID WP_000729704.1
Coordinates 3725730..3725990 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ158_RS17970 (3721513) 3721513..3722298 - 786 WP_000207574.1 putative lateral flagellar export/assembly protein LafU -
LQ158_RS17975 (3722270) 3722270..3723982 + 1713 Protein_3528 flagellar biosynthesis protein FlhA -
LQ158_RS17980 (3724087) 3724087..3724365 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
LQ158_RS17985 (3724358) 3724358..3724714 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
LQ158_RS17990 (3724771) 3724771..3725544 - 774 WP_000543897.1 C40 family peptidase -
LQ158_RS17995 (3725730) 3725730..3725990 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
LQ158_RS18000 (3725993) 3725993..3726271 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
LQ158_RS18005 (3726427) 3726427..3727167 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
LQ158_RS18010 (3727138) 3727138..3727905 - 768 WP_000333380.1 class II glutamine amidotransferase -
LQ158_RS18015 (3728111) 3728111..3728689 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T295689 WP_000615976.1 NZ_OW849377:3725993-3726271 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT295689 WP_000729704.1 NZ_OW849377:3725730-3725990 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References