Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 594547..595346 Replicon chromosome
Accession NZ_OW849377
Organism Escherichia coli isolate 162

Toxin (Protein)


Gene name yhaV Uniprot ID U9XVR9
Locus tag LQ158_RS02920 Protein ID WP_000347267.1
Coordinates 594547..595011 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LQ158_RS02925 Protein ID WP_001307405.1
Coordinates 595011..595346 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ158_RS02890 (589548) 589548..589982 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
LQ158_RS02895 (590000) 590000..590878 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LQ158_RS02900 (590868) 590868..591647 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LQ158_RS02905 (591658) 591658..592131 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LQ158_RS02910 (592154) 592154..593434 - 1281 WP_230133043.1 tagatose-bisphosphate aldolase subunit KbaZ -
LQ158_RS02915 (593683) 593683..594492 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LQ158_RS02920 (594547) 594547..595011 - 465 WP_000347267.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LQ158_RS02925 (595011) 595011..595346 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LQ158_RS02930 (595495) 595495..597066 - 1572 WP_001273741.1 galactarate dehydratase -
LQ158_RS02935 (597441) 597441..598775 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
LQ158_RS02940 (598791) 598791..599561 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 594547..606002 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17807.17 Da        Isoelectric Point: 9.4947

>T295676 WP_000347267.1 NZ_OW849377:c595011-594547 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT295676 WP_001307405.1 NZ_OW849377:c595346-595011 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XTR4

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References