Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 33039..33769 Replicon chromosome
Accession NZ_OW849195
Organism Enterobacter cloacae isolate 125

Toxin (Protein)


Gene name higB Uniprot ID A0A2W0GI63
Locus tag LQ145_RS00155 Protein ID WP_023331686.1
Coordinates 33039..33353 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LQ145_RS00160 Protein ID WP_023331687.1
Coordinates 33350..33769 (+) Length 140 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ145_RS00135 (AI2762V1_0028) 28371..29555 - 1185 WP_032629627.1 multidrug efflux MFS transporter EmrD -
LQ145_RS00140 (AI2762V1_0029) 29731..30564 - 834 WP_023331683.1 EamA family transporter -
LQ145_RS00145 (AI2762V1_0030) 30627..31073 - 447 WP_023331684.1 GNAT family N-acetyltransferase -
LQ145_RS00150 (AI2762V1_0032) 31471..32691 - 1221 WP_000343760.1 ISL3-like element ISKox3 family transposase -
LQ145_RS00155 (AI2762V1_0033) 33039..33353 + 315 WP_023331686.1 type II toxin-antitoxin system HigB family toxin Toxin
LQ145_RS00160 (AI2762V1_0034) 33350..33769 + 420 WP_023331687.1 helix-turn-helix domain-containing protein Antitoxin
LQ145_RS00165 33919..34017 + 99 WP_023331688.1 ilvB operon leader peptide IvbL -
LQ145_RS00170 (AI2762V1_0035) 34124..35812 + 1689 WP_023331689.1 acetolactate synthase large subunit -
LQ145_RS00175 (AI2762V1_0036) 35816..36103 + 288 WP_010426528.1 acetolactate synthase small subunit -
LQ145_RS00180 (AI2762V1_0037) 36186..36779 + 594 WP_014881931.1 transcriptional regulator UhpA -
LQ145_RS00185 (AI2762V1_0038) 36776..38281 + 1506 WP_023331690.1 signal transduction histidine-protein kinase/phosphatase UhpB -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 31471..32691 1220


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 105 a.a.        Molecular weight: 12441.59 Da        Isoelectric Point: 10.2825

>T295663 WP_023331686.1 NZ_OW849195:33039-33353 [Enterobacter cloacae]
MHLISMKAILDAVIQFPQHREELLFLGRTIEKSHCTTPVALRKIFPTLDNLKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Download         Length: 315 bp


Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15561.18 Da        Isoelectric Point: 5.6761

>AT295663 WP_023331687.1 NZ_OW849195:33350-33769 [Enterobacter cloacae]
MMIVADAMKATHALVAAVPLLGEHPNEKDYQDALELVEYLLMNEPGSPLLDIVCARIRRYEANRPEIVALRQEMESVPVG
IAVLRTLMDQYKLTISDFQDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Download         Length: 420 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A2W0GI63


Antitoxin

Source ID Structure

References