Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2048116..2048979 Replicon chromosome
Accession NZ_OW849082
Organism Citrobacter freundii isolate 8

Toxin (Protein)


Gene name yhaV Uniprot ID A0A7W3E442
Locus tag LQ154_RS10040 Protein ID WP_044702672.1
Coordinates 2048116..2048610 (-) Length 165 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A5B0SRB7
Locus tag LQ154_RS10045 Protein ID WP_044702675.1
Coordinates 2048635..2048979 (-) Length 115 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LQ154_RS10025 (2045614) 2045614..2046450 + 837 Protein_1963 Tn3 family transposase -
LQ154_RS10030 (2046447) 2046447..2047043 - 597 WP_230129643.1 hypothetical protein -
LQ154_RS10035 (2047141) 2047141..2047389 - 249 WP_044702684.1 hypothetical protein -
LQ154_RS10040 (2048116) 2048116..2048610 - 495 WP_044702672.1 type II toxin-antitoxin system YhaV family toxin Toxin
LQ154_RS10045 (2048635) 2048635..2048979 - 345 WP_044702675.1 type II toxin-antitoxin system PrlF family antitoxin Antitoxin
LQ154_RS10050 (2049416) 2049416..2049646 - 231 WP_044702671.1 hypothetical protein -
LQ154_RS10055 (2049637) 2049637..2049900 - 264 WP_044702670.1 DUF1380 family protein -
LQ154_RS10060 (2050007) 2050007..2050288 - 282 WP_044702669.1 hypothetical protein -
LQ154_RS10065 (2050436) 2050436..2050954 - 519 WP_044702667.1 hypothetical protein -
LQ154_RS10070 (2050944) 2050944..2051318 - 375 WP_044702665.1 hypothetical protein -
LQ154_RS10075 (2051328) 2051328..2051657 - 330 WP_044702664.1 hypothetical protein -
LQ154_RS10080 (2051671) 2051671..2051940 - 270 WP_044702662.1 hypothetical protein -
LQ154_RS10085 (2052049) 2052049..2052480 - 432 WP_049002914.1 antirestriction protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island blaTEM-1A / aac(3)-IIa / blaTEM-1B - 2031035..2069815 38780


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(11-147)

Antitoxin

PrlF antitoxin

(16-113)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 19076.90 Da        Isoelectric Point: 8.2557

>T295636 WP_044702672.1 NZ_OW849082:c2048610-2048116 [Citrobacter freundii]
MEYLEINGWKICFHKCFLDQITELAKKVAELKAAKPSEYHTKKETKLLRAIERVIEEWIASDPLNPQYRQGETLGDEYKH
WFRVKFLQQFRLFFRCSQEHKTIIIGWVNDFGTLRAYGSKTDAYKVFAAMLDAGNPPDDWDKLLNAATKATATASIPDFL
TRQP

Download         Length: 495 bp

Antitoxin


Download         Length: 115 a.a.        Molecular weight: 12537.26 Da        Isoelectric Point: 4.6787

>AT295636 WP_044702675.1 NZ_OW849082:c2048979-2048635 [Citrobacter freundii]
MATQILNSADVSLRAESRLTERSQTTIPAAIRDALHLKPGESIHYTLLAGGKVIMSRKDEEQDDPVVSQFLAFLEKDMKK
NPQNIAPVPAFFWESIKSLTAGVEVDLDAPLTDS

Download         Length: 345 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W3E442

Antitoxin

Source ID Structure
AlphaFold DB A0A5B0SRB7

References