Detailed information of TA system TA029557

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 905626..906416
• Replicon: chromosome
• Accession: NC_021505
• Organism: Pseudomonas putida NBRC 14164

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: PP4_RS03985
• Protein ID: WP_016497991.1
• Coordinates: 905626..906093 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: I7C311
• Locus tag: PP4_RS03990
• Protein ID: WP_014859917.1
• Coordinates: 906090..906416 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PP4_RS03965	900789..902318	+	1530	WP_016497988.1	efflux transporter outer membrane subunit	-
PP4_RS03970	902315..904492	+	2178	WP_041167558.1	FUSC family protein	-
PP4_RS03975	904482..904682	+	201	WP_009685040.1	DUF1656 domain-containing protein	-
PP4_RS03980	904693..905553	+	861	WP_016497990.1	HlyD family secretion protein	-
PP4_RS03985	905626..906093	-	468	WP_016497991.1	type II toxin-antitoxin system YhaV family toxin	Toxin
PP4_RS03990	906090..906416	-	327	WP_014859917.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
PP4_RS03995	906509..906955	-	447	WP_016497992.1	universal stress protein	-
PP4_RS04000	907226..908131	+	906	WP_016497993.1	LysR family transcriptional regulator	-
PP4_RS04005	908248..909789	+	1542	WP_016497994.1	MFS transporter	-
PP4_RS04010	909818..910879	+	1062	WP_016497995.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (17-152)

Antitoxin

• PrlF antitoxin (8-106)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17879.27 Da
• Isoelectric Point: 9.6727

>T29557 WP_016497991.1 NC_021505:c906093-905626 [Pseudomonas putida NBRC 14164]
MSDASRKPLVIHGWTVIAHPLFLAKLDALSAQVEAQRDKDPTGYTRRNAFKRLAAIRRLAFDVIPQDPTKPEYRQGATLG
GDHKHWFRAKFFQQYRLFFRYHTPSRIIVLAWVNDESSKRACESSDDAYKVFQKMLHGGHPPDDWDQLLQEAAAD

Nucleotide

• Length: 468 bp

>T29557 NC_021505:c906093-905626 [Pseudomonas putida NBRC 14164]
ATGAGTGATGCGAGCAGGAAGCCTCTGGTTATTCATGGATGGACTGTCATTGCCCACCCGCTGTTCCTGGCCAAACTGGA
CGCGCTAAGCGCTCAAGTCGAGGCTCAACGGGACAAAGACCCAACGGGTTATACGAGAAGAAATGCCTTTAAAAGGCTGG
CCGCCATCCGGCGGTTGGCGTTCGATGTAATCCCTCAAGACCCGACCAAACCCGAATACCGGCAAGGGGCAACACTTGGC
GGTGACCACAAGCACTGGTTCAGAGCAAAGTTCTTCCAGCAGTACCGGTTGTTCTTCCGCTACCACACCCCCAGCAGGAT
CATCGTGCTGGCCTGGGTCAATGACGAGTCGAGCAAGCGGGCCTGCGAAAGTAGCGACGATGCTTACAAGGTCTTTCAAA
AGATGTTGCATGGCGGCCACCCACCGGACGATTGGGACCAGTTGCTGCAAGAGGCTGCCGCAGATTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11919.40 Da
• Isoelectric Point: 4.3722

>AT29557 WP_014859917.1 NC_021505:c906416-906090 [Pseudomonas putida NBRC 14164]
MAATFDVESTLTDRYQTTVPETVRRALGLKKRDKIHYSIRPDGGVILTRAEATEDDPVLGKFLDFLAHDIATNPQRLQVV
DAGLIARLDKLVGDAEVDLDQPLSADDE

Nucleotide

• Length: 327 bp

>AT29557 NC_021505:c906416-906090 [Pseudomonas putida NBRC 14164]
ATGGCTGCCACCTTCGACGTCGAGTCCACGCTGACCGATCGCTATCAAACCACTGTTCCCGAAACGGTGCGGCGCGCCTT
GGGCTTGAAAAAACGTGACAAGATCCATTATTCGATTCGCCCCGACGGTGGGGTGATACTCACCCGCGCAGAGGCAACCG
AGGACGATCCGGTACTGGGCAAATTTCTGGATTTCCTGGCGCACGATATCGCTACCAACCCTCAACGGCTGCAGGTTGTC
GATGCTGGCCTCATCGCGCGCCTCGACAAGCTTGTCGGCGACGCGGAGGTCGACCTGGATCAACCTCTGTCGGCGGACGA
TGAATGA

Structures

Antitoxin

• AlphaFold DB: I7C311