Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3319618..3320360 Replicon chromosome
Accession NC_021500
Organism Enterobacter sp. R4-368

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag H650_RS15625 Protein ID WP_020456090.1
Coordinates 3319618..3319929 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag H650_RS15630 Protein ID WP_020456091.1
Coordinates 3319926..3320360 (+) Length 145 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H650_RS15600 3315066..3315842 + 777 WP_020456086.1 hypothetical protein -
H650_RS15605 3315826..3316293 + 468 WP_110093693.1 hypothetical protein -
H650_RS15610 3316465..3318096 + 1632 WP_020456088.1 Na/Pi cotransporter family protein -
H650_RS15615 3318234..3319097 + 864 WP_020456089.1 23S rRNA pseudouridine(2604) synthase RluF -
H650_RS15620 3319098..3319370 - 273 WP_017459411.1 DUF3811 domain-containing protein -
H650_RS15625 3319618..3319929 + 312 WP_020456090.1 type II toxin-antitoxin system HigB family toxin Toxin
H650_RS15630 3319926..3320360 + 435 WP_020456091.1 helix-turn-helix domain-containing protein Antitoxin
H650_RS15635 3320397..3321338 - 942 WP_020456092.1 ketopantoate/pantoate/pantothenate transporter PanS -
H650_RS15640 3321588..3323174 - 1587 WP_020456093.1 RNA repair transcriptional activator RtcR -
H650_RS15645 3323645..3324787 + 1143 WP_020456095.1 slipin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(81-135)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12202.10 Da        Isoelectric Point: 9.7491

>T29548 WP_020456090.1 NC_021500:3319618-3319929 [Enterobacter sp. R4-368]
MHVISRRPFDNAAKDFPNHTLALDAVYKILKSTVYQNPDALRVEFPSLDKMKYRDKWWVINVGGNHLRVLFFADFESAKI
FVKHIVTHAECDKLIRHYRETKE

Download         Length: 312 bp

>T29548 NC_021500:3319618-3319929 [Enterobacter sp. R4-368]
ATGCACGTTATATCAAGGAGGCCGTTCGATAACGCGGCTAAAGATTTTCCAAACCACACGCTTGCGCTGGACGCGGTTTA
CAAGATCCTGAAAAGCACTGTTTACCAGAATCCAGACGCGTTGCGAGTAGAGTTCCCAAGCCTGGACAAGATGAAATACA
GGGATAAATGGTGGGTTATCAATGTCGGTGGCAACCATTTGAGGGTGCTGTTTTTCGCAGACTTTGAATCAGCAAAAATA
TTTGTCAAACATATCGTGACCCATGCCGAGTGTGACAAATTGATAAGACATTATCGGGAGACCAAAGAATGA

Antitoxin


Download         Length: 145 a.a.        Molecular weight: 15977.17 Da        Isoelectric Point: 5.5556

>AT29548 WP_020456091.1 NC_021500:3319926-3320360 [Enterobacter sp. R4-368]
MIEDAIKAADELVKQVPFLGNNPGKEEYEQALEMVERLLMHAPDSSLAALLTAKIEHYENNDPELAAFNARIAALPRGVA
ALRVLMDQHGLNQSSFRDEIGQRSLVSRILNGERNLTVDHIRALAKRFNVSTDVFIEPAHHIAG

Download         Length: 435 bp

>AT29548 NC_021500:3319926-3320360 [Enterobacter sp. R4-368]
ATGATTGAGGATGCGATCAAGGCCGCTGACGAGCTGGTGAAACAGGTGCCTTTTTTGGGAAATAACCCCGGCAAAGAGGA
GTATGAGCAAGCGCTGGAGATGGTTGAACGGTTGCTCATGCATGCGCCGGATAGCTCACTGGCCGCGCTGCTTACCGCAA
AAATAGAGCACTATGAAAATAACGACCCGGAGCTGGCGGCGTTTAACGCGCGTATCGCGGCACTGCCACGCGGTGTTGCC
GCTTTACGGGTTTTGATGGATCAGCACGGATTAAATCAGTCTTCGTTTCGGGATGAAATCGGCCAGCGCTCCCTGGTGAG
CCGTATTCTTAATGGCGAACGCAATCTGACTGTCGACCATATCCGAGCGCTGGCGAAACGCTTTAATGTGTCGACCGACG
TGTTTATTGAGCCAGCCCATCACATCGCGGGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References