Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3198728..3199398 Replicon chromosome
Accession NZ_OW052302
Organism Mycobacterium tuberculosis strain Lineage 3.1.1 isolate Mycobacterium tuberculosis

Toxin (Protein)


Gene name higB Uniprot ID O53332
Locus tag MR176_RS14745 Protein ID WP_003899954.1
Coordinates 3199054..3199398 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID G0THF6
Locus tag MR176_RS14740 Protein ID WP_003899955.1
Coordinates 3198728..3199057 (-) Length 110 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MR176_RS14710 3194143..3195177 + 1035 WP_003416786.1 IS30 family transposase -
MR176_RS14715 3195204..3195389 - 186 WP_015630737.1 hypothetical protein -
MR176_RS14720 3195424..3195633 - 210 WP_003416778.1 hypothetical protein -
MR176_RS14725 3195801..3197066 + 1266 WP_003902423.1 hypothetical protein -
MR176_RS14730 3197226..3197846 - 621 WP_003899957.1 RES family NAD+ phosphorylase -
MR176_RS14735 3197843..3198190 - 348 WP_003899956.1 DUF2384 domain-containing protein -
MR176_RS14740 3198728..3199057 - 330 WP_003899955.1 XRE family transcriptional regulator Antitoxin
MR176_RS14745 3199054..3199398 - 345 WP_003899954.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MR176_RS14750 3199629..3200081 + 453 WP_003899953.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
MR176_RS14755 3200084..3200518 + 435 WP_003899952.1 type II toxin-antitoxin system VapC family toxin -
MR176_RS14760 3200865..3202154 - 1290 WP_003416640.1 ATP-binding protein -
MR176_RS14765 3202442..3202735 - 294 WP_003416635.1 hypothetical protein -
MR176_RS14770 3202748..3202959 - 212 Protein_2921 (R)-hydratase -
MR176_RS14775 3202975..3203490 - 516 WP_003899950.1 nitroreductase family deazaflavin-dependent oxidoreductase -
MR176_RS14780 3203465..3204325 - 861 WP_003416628.1 alpha/beta hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-91)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12692.50 Da        Isoelectric Point: 5.6920

>T295243 WP_003899954.1 NZ_OW052302:c3199398-3199054 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Download         Length: 345 bp

Antitoxin


Download         Length: 110 a.a.        Molecular weight: 11802.46 Da        Isoelectric Point: 7.4051

>AT295243 WP_003899955.1 NZ_OW052302:c3199057-3198728 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAALGGHLRIVAEFGENTVELTA

Download         Length: 330 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBK2

Antitoxin

Source ID Structure
PDB 6LTY
PDB 6LTZ
AlphaFold DB G0THF6

References