Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 51173..52092 Replicon chromosome
Accession NZ_OW052302
Organism Mycobacterium tuberculosis strain Lineage 3.1.1 isolate Mycobacterium tuberculosis

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag MR176_RS00180 Protein ID WP_003900449.1
Coordinates 51173..51778 (+) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag MR176_RS00185 Protein ID WP_003410124.1
Coordinates 51787..52092 (+) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MR176_RS00160 48661..49557 + 897 WP_003410136.1 hypothetical protein -
MR176_RS00165 49764..50000 + 237 WP_003410133.1 hypothetical protein -
MR176_RS00170 49997..50629 + 633 WP_003913310.1 hypothetical protein -
MR176_RS00175 50789..51148 + 360 WP_003410131.1 hypothetical protein -
MR176_RS00180 51173..51778 + 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MR176_RS00185 51787..52092 + 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
MR176_RS00190 52177..52476 + 300 WP_003410120.1 hypothetical protein -
MR176_RS00195 52492..52908 - 417 WP_003410114.1 hypothetical protein -
MR176_RS00200 52898..53617 - 720 WP_003410108.1 DUF433 domain-containing protein -
MR176_RS00205 53859..54899 - 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
MR176_RS00210 54896..55471 - 576 WP_003410100.1 hypothetical protein -
MR176_RS00215 55825..57081 + 1257 WP_016720149.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T295201 WP_003900449.1 NZ_OW052302:51173-51778 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT295201 WP_003410124.1 NZ_OW052302:51787-52092 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References