Detailed information of TA system TA029516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 166115..166793
• Replicon: chromosome
• Accession: NC_021287
• Organism: Burkholderia sp. RPE64

Toxin (Protein)

• Gene name: higB
• Uniprot ID: R4WVJ1
• Locus tag: BRPE64_RS00780
• Protein ID: WP_016344085.1
• Coordinates: 166443..166793 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BRPE64_RS00775
• Protein ID: WP_044041029.1
• Coordinates: 166115..166450 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BRPE64_RS00750	162538..163416	+	879	WP_016344079.1	hypothetical protein	-
BRPE64_RS00755	163567..164049	+	483	WP_016344080.1	D-glycero-beta-D-manno-heptose 1-phosphate adenylyltransferase	-
BRPE64_RS00760	164063..164302	-	240	WP_016344081.1	hypothetical protein	-
BRPE64_RS00765	164340..165161	-	822	WP_016344082.1	type III pantothenate kinase	-
BRPE64_RS00770	165158..166060	-	903	WP_016344083.1	biotin--[acetyl-CoA-carboxylase] ligase	-
BRPE64_RS00775	166115..166450	-	336	WP_044041029.1	helix-turn-helix domain-containing protein	Antitoxin
BRPE64_RS00780	166443..166793	-	351	WP_016344085.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
BRPE64_RS00785	166977..168104	+	1128	WP_016344086.1	ABC transporter permease	-
BRPE64_RS00790	168110..168988	+	879	WP_016344087.1	ABC transporter ATP-binding protein	-
BRPE64_RS00795	169046..170005	+	960	WP_016344088.1	MCE family protein	-
BRPE64_RS00800	170037..170663	+	627	WP_016344089.1	membrane integrity-associated transporter subunit PqiC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (22-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-106)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13179.39 Da
• Isoelectric Point: 10.7255

>T29516 WP_016344085.1 NC_021287:c166793-166443 [Caballeronia insecticola]
MLTSADVDAWAITYYSERIAREISELPVGIFAGYLRLLSALKQHGPDLGMPYSRSLGDKLFELRPAGREGIARVFYGAMQ
GKRIIMLHSFIKKTQRTPPKELRLARNRLMEAQRNG

Nucleotide

• Length: 351 bp

>T29516 NC_021287:c166793-166443 [Caballeronia insecticola]
ATGCTAACATCCGCCGATGTCGATGCCTGGGCGATTACGTACTACAGCGAGCGGATCGCGCGCGAGATTTCCGAGTTGCC
GGTCGGAATTTTTGCCGGCTATCTGCGCCTCCTGTCCGCGCTGAAACAGCATGGTCCCGACCTGGGCATGCCGTATTCCA
GATCGCTGGGCGACAAGCTTTTCGAGCTTCGGCCAGCAGGACGGGAAGGGATCGCTCGTGTGTTCTACGGCGCAATGCAG
GGAAAGCGAATCATCATGCTGCACTCGTTCATCAAGAAAACGCAAAGGACGCCGCCCAAGGAACTGCGCCTTGCCCGGAA
TCGCTTAATGGAGGCTCAACGGAATGGCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12075.84 Da
• Isoelectric Point: 9.4469

>AT29516 WP_044041029.1 NC_021287:c166450-166115 [Caballeronia insecticola]
MAKTRWQKELAEQYVPVSHDDGKVARQLAANPELKAAYDALAPEYEALDALLHARKEAGLTQAQIAERMGTTASAVARLE
GSLISEKHSPSISTLRKYAKACGKTLRISLV

Nucleotide

• Length: 336 bp

>AT29516 NC_021287:c166450-166115 [Caballeronia insecticola]
ATGGCTAAGACGCGTTGGCAAAAGGAATTGGCGGAACAGTACGTTCCTGTGTCGCATGACGACGGCAAGGTCGCCCGGCA
GCTGGCCGCCAACCCCGAACTCAAGGCCGCATACGATGCACTCGCACCTGAATACGAAGCACTCGACGCCCTGCTTCACG
CCCGCAAGGAAGCGGGTCTGACACAAGCGCAAATCGCCGAGCGCATGGGAACGACCGCATCCGCAGTCGCGCGGCTCGAA
GGGTCGCTGATCAGCGAGAAGCACTCACCATCCATTTCGACGCTGCGTAAATACGCGAAGGCCTGCGGCAAAACACTGCG
CATCTCACTGGTGTAA

Structures

Toxin

• AlphaFold DB: R4WVJ1