Detailed information of TA system TA029514

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2699057..2699685
• Replicon: chromosome
• Accession: NC_021286
• Organism: Idiomarina loihiensis GSL 199

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q5QV89
• Locus tag: K734_RS12890
• Protein ID: WP_011235744.1
• Coordinates: 2699057..2699350 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K734_RS12895
• Protein ID: WP_011235745.1
• Coordinates: 2699362..2699685 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K734_RS12865	2695388..2696044	-	657	WP_011235739.1	epoxyqueuosine reductase QueH	-
K734_RS12870	2696171..2696401	+	231	WP_011235740.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
K734_RS12875	2696398..2696847	+	450	WP_011235741.1	type II toxin-antitoxin system VapC family toxin	-
K734_RS12880	2696862..2697569	-	708	WP_011235742.1	ZIP family metal transporter	-
K734_RS12885	2697572..2698852	-	1281	WP_011235743.1	deoxyguanosinetriphosphate triphosphohydrolase family protein	-
K734_RS12890	2699057..2699350	+	294	WP_011235744.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K734_RS12895	2699362..2699685	+	324	WP_011235745.1	HigA family addiction module antidote protein	Antitoxin
K734_RS12900	2699707..2700195	-	489	WP_011235746.1	hypothetical protein	-
K734_RS12905	2700387..2701055	+	669	WP_011235747.1	hypothetical protein	-
K734_RS12910	2701052..2701450	-	399	WP_011235748.1	type II toxin-antitoxin system YafO family toxin	-
K734_RS12915	2701447..2701740	-	294	WP_011235749.1	type I toxin-antitoxin system antitoxin YafN	-
K734_RS12920	2701834..2702976	-	1143	WP_011235750.1	alpha/beta hydrolase	-
K734_RS12925	2703107..2703622	+	516	WP_011235751.1	DNA mismatch endonuclease Vsr	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11509.13 Da
• Isoelectric Point: 7.2739

>T29514 WP_011235744.1 NC_021286:2699057-2699350 [Idiomarina loihiensis GSL 199]
MAVTFRDDWLERFYEDDISHKKIPKIIKGALFRKLEILDAATQESDLRVPPGNRFEYLKGKLSGYCSIRVNKQYRLIFHW
EDGIAQDTYLDPHVYQS

Nucleotide

• Length: 294 bp

>T29514 NC_021286:2699057-2699350 [Idiomarina loihiensis GSL 199]
ATGGCCGTTACATTCAGGGACGATTGGCTAGAACGATTCTACGAAGATGATATCAGCCACAAGAAAATACCGAAGATCAT
CAAAGGAGCGCTCTTCAGGAAGCTCGAAATTTTGGATGCTGCAACGCAGGAGTCAGATTTAAGGGTTCCCCCAGGTAATC
GCTTCGAATATTTAAAAGGAAAACTTTCGGGGTATTGTTCTATCCGGGTTAACAAACAGTATCGACTTATTTTCCACTGG
GAAGACGGTATCGCCCAAGATACATACCTGGATCCACACGTTTACCAAAGTTAA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 12351.33 Da
• Isoelectric Point: 10.0417

>AT29514 WP_011235745.1 NC_021286:2699362-2699685 [Idiomarina loihiensis GSL 199]
MRDTKRRPVSVGQMLITEFLEPMNIEIKELADAMRVHRNTLSRIVHDKGALTAPMAIKLAAALGNTPEFWLNIQHAVEIW
DVRHRAYEQEAENVKRLKPHAKPYQQA

Nucleotide

• Length: 324 bp

>AT29514 NC_021286:2699362-2699685 [Idiomarina loihiensis GSL 199]
ATGAGAGATACAAAACGTCGCCCGGTATCCGTTGGCCAGATGCTAATAACCGAGTTTCTTGAACCGATGAACATTGAAAT
AAAAGAGCTCGCTGATGCTATGAGAGTACACAGAAACACGCTCAGCCGAATTGTTCATGACAAAGGAGCCTTGACCGCAC
CTATGGCTATTAAATTAGCTGCCGCTTTGGGTAACACCCCGGAGTTCTGGTTGAACATTCAGCACGCCGTCGAAATCTGG
GACGTACGCCACCGGGCATACGAACAAGAAGCTGAAAACGTAAAGCGCTTGAAACCTCACGCTAAGCCTTATCAGCAGGC
TTGA

Structures

Toxin

• AlphaFold DB: Q5QV89