Detailed information of TA system TA029502

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 8306016..8306721
• Replicon: chromosome
• Accession: NC_021252
• Organism: Amycolatopsis orientalis HCCB10007

Toxin (Protein)

• Gene name: higB
• Uniprot ID: R4TIL1
• Locus tag: AORI_RS78500
• Protein ID: WP_016337758.1
• Coordinates: 8306356..8306721 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AORI_RS78495
• Protein ID: WP_016337757.1
• Coordinates: 8306016..8306354 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AORI_RS78480	8301936..8303351	+	1416	WP_016337754.1	M1 family metallopeptidase	-
AORI_RS78485	8303555..8304190	+	636	WP_016337755.1	pyroglutamyl-peptidase I	-
AORI_RS78490	8304191..8305954	-	1764	WP_016337756.1	Hsp70 family protein	-
AORI_RS78495	8306016..8306354	-	339	WP_016337757.1	XRE family transcriptional regulator	Antitoxin
AORI_RS78500	8306356..8306721	-	366	WP_016337758.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
AORI_RS78505	8307094..8309652	-	2559	WP_016337759.1	ATP-dependent Clp protease ATP-binding subunit	-
AORI_RS78510	8310059..8310817	+	759	WP_123799180.1	(2Fe-2S)-binding protein	-
AORI_RS78515	8310814..8311395	-	582	WP_016337761.1	TIGR03086 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (22-112)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-92)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 14242.22 Da
• Isoelectric Point: 6.4923

>T29502 WP_016337758.1 NC_021252:c8306721-8306356 [Amycolatopsis keratiniphila]
MQWEIELHEEVDRWFAALCREEPRTADRVEEAIDMLAREGPRLGRPLVDRISGSRRHNLKELRPASAGHTEIRILFVFDP
VRRAVLLVAGDKAGNWKRWYDINIPIAEERYRRHLDAMEEG

Nucleotide

• Length: 366 bp

>T29502 NC_021252:c8306721-8306356 [Amycolatopsis keratiniphila]
ATGCAGTGGGAGATCGAACTTCACGAGGAAGTGGATCGCTGGTTCGCGGCGCTGTGTCGGGAAGAACCGCGTACGGCCGA
CCGGGTCGAAGAGGCGATCGACATGCTGGCTCGTGAAGGTCCCCGGCTCGGGCGGCCACTGGTCGACCGGATCAGCGGCT
CGCGAAGACACAACTTGAAAGAGTTACGCCCGGCGTCGGCCGGGCACACCGAGATCAGGATCCTGTTCGTCTTCGACCCG
GTGCGCCGGGCCGTGCTCCTGGTAGCCGGTGACAAGGCCGGAAACTGGAAACGGTGGTACGACATCAACATCCCGATCGC
GGAGGAGCGATACCGGCGGCATCTGGACGCGATGGAGGAAGGCTGA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 12475.10 Da
• Isoelectric Point: 5.9250

>AT29502 WP_016337757.1 NC_021252:c8306354-8306016 [Amycolatopsis keratiniphila]
MGRSWKEVKAEKLAADREAGRDVEAARAEAREATQAYVLGFRLSRLREDIGLSQAEVAKRMGVSQPRISQLEQGDPSQME
LDTLRRYIVALGGRMRVVADFDDHDVTISTAV

Nucleotide

• Length: 339 bp

>AT29502 NC_021252:c8306354-8306016 [Amycolatopsis keratiniphila]
ATGGGTCGGTCCTGGAAAGAGGTCAAGGCCGAAAAGCTCGCCGCGGACCGTGAAGCCGGTCGCGACGTCGAAGCCGCGCG
TGCCGAGGCGCGAGAAGCCACTCAGGCCTACGTTCTCGGCTTCCGGCTGTCGCGACTGCGTGAGGACATCGGGCTGAGCC
AGGCCGAGGTCGCCAAGCGAATGGGCGTCAGCCAGCCGCGGATCAGCCAGCTCGAACAGGGTGATCCGAGCCAGATGGAG
TTGGACACGCTCCGCAGGTACATCGTCGCGTTGGGCGGCCGCATGCGCGTGGTCGCCGATTTCGACGATCACGACGTCAC
CATTTCCACCGCCGTCTGA

Structures

Toxin

• AlphaFold DB: R4TIL1