Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4493753..4494355 Replicon chromosome
Accession NZ_OU701452
Organism Escherichia coli isolate CNR65D6

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag LWS60_RS21655 Protein ID WP_000897305.1
Coordinates 4493753..4494064 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LWS60_RS21660 Protein ID WP_000356395.1
Coordinates 4494065..4494355 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LWS60_RS21630 (4488795) 4488795..4489232 + 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -
LWS60_RS21635 (4489277) 4489277..4490218 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
LWS60_RS21640 (4490634) 4490634..4491521 + 888 Protein_4233 hypothetical protein -
LWS60_RS21645 (4491534) 4491534..4492448 + 915 WP_109553727.1 transposase -
LWS60_RS21650 (4492616) 4492616..4493524 - 909 WP_001550353.1 alpha/beta hydrolase -
LWS60_RS21655 (4493753) 4493753..4494064 + 312 WP_000897305.1 hypothetical protein Toxin
LWS60_RS21660 (4494065) 4494065..4494355 + 291 WP_000356395.1 NadS family protein Antitoxin
LWS60_RS21665 (4494440) 4494440..4494661 + 222 WP_001550354.1 hypothetical protein -
LWS60_RS21670 (4494713) 4494713..4494991 + 279 WP_001315112.1 hypothetical protein -
LWS60_RS21675 (4495410) 4495410..4495628 + 219 WP_001295676.1 CopG family transcriptional regulator -
LWS60_RS21680 (4495847) 4495847..4496089 + 243 WP_001309881.1 CopG family transcriptional regulator -
LWS60_RS21685 (4496271) 4496271..4497200 - 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
LWS60_RS21690 (4497197) 4497197..4497832 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
LWS60_RS21695 (4497829) 4497829..4498731 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T294878 WP_000897305.1 NZ_OU701452:4493753-4494064 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 10.8907

>AT294878 WP_000356395.1 NZ_OU701452:4494065-4494355 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References