Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3661891..3662690 Replicon chromosome
Accession NZ_OU701452
Organism Escherichia coli isolate CNR65D6

Toxin (Protein)


Gene name yhaV Uniprot ID F0JQM9
Locus tag LWS60_RS17680 Protein ID WP_000347270.1
Coordinates 3662226..3662690 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID A0A0V9H1L4
Locus tag LWS60_RS17675 Protein ID WP_001551693.1
Coordinates 3661891..3662226 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LWS60_RS17660 (3657676) 3657676..3658446 - 771 WP_001551692.1 2-dehydro-3-deoxyglucarate aldolase -
LWS60_RS17665 (3658462) 3658462..3659796 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
LWS60_RS17670 (3660171) 3660171..3661742 + 1572 WP_001273738.1 galactarate dehydratase -
LWS60_RS17675 (3661891) 3661891..3662226 + 336 WP_001551693.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LWS60_RS17680 (3662226) 3662226..3662690 + 465 WP_000347270.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LWS60_RS17685 (3662745) 3662745..3663554 - 810 WP_106106567.1 aga operon transcriptional regulator AgaR -
LWS60_RS17690 (3663803) 3663803..3665083 + 1281 WP_001551694.1 tagatose-bisphosphate aldolase subunit KbaZ -
LWS60_RS17695 (3665106) 3665106..3665579 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
LWS60_RS17700 (3665590) 3665590..3666369 + 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
LWS60_RS17705 (3666359) 3666359..3667237 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
LWS60_RS17710 (3667255) 3667255..3667689 + 435 WP_000948837.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3653129..3662690 9561


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T294876 WP_000347270.1 NZ_OU701452:3662226-3662690 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12347.87 Da        Isoelectric Point: 4.6890

>AT294876 WP_001551693.1 NZ_OU701452:3661891-3662226 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9NQ90

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9H1L4

References