Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3070650..3071377 Replicon chromosome
Accession NZ_OU701452
Organism Escherichia coli isolate CNR65D6

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag LWS60_RS14785 Protein ID WP_000547555.1
Coordinates 3071066..3071377 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LWS60_RS14780 Protein ID WP_000126294.1
Coordinates 3070650..3071069 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LWS60_RS14760 (3065735) 3065735..3066262 - 528 WP_001078777.1 electron transport protein HydN -
LWS60_RS14765 (3066411) 3066411..3067421 - 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
LWS60_RS14770 (3067681) 3067681..3069138 + 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
LWS60_RS14775 (3069147) 3069147..3070571 + 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
LWS60_RS14780 (3070650) 3070650..3071069 - 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
LWS60_RS14785 (3071066) 3071066..3071377 - 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
LWS60_RS14790 (3071544) 3071544..3072014 - 471 WP_001551542.1 hydrogenase maturation peptidase HycI -
LWS60_RS14795 (3072007) 3072007..3072417 - 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
LWS60_RS14800 (3072414) 3072414..3073181 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
LWS60_RS14805 (3073181) 3073181..3073723 - 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
LWS60_RS14810 (3073733) 3073733..3075442 - 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T294871 WP_000547555.1 NZ_OU701452:c3071377-3071066 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT294871 WP_000126294.1 NZ_OU701452:c3071069-3070650 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References