Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4966128..4966730 Replicon chromosome
Accession NZ_OU701449
Organism Escherichia coli isolate CNR85I8

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag LWS57_RS23995 Protein ID WP_000897305.1
Coordinates 4966419..4966730 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LWS57_RS23990 Protein ID WP_000356395.1
Coordinates 4966128..4966418 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LWS57_RS23955 (4961752) 4961752..4962654 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
LWS57_RS23960 (4962651) 4962651..4963286 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
LWS57_RS23965 (4963283) 4963283..4964212 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
LWS57_RS23970 (4964394) 4964394..4964636 - 243 WP_001309881.1 CopG family transcriptional regulator -
LWS57_RS23975 (4964855) 4964855..4965073 - 219 WP_001295676.1 CopG family transcriptional regulator -
LWS57_RS23980 (4965492) 4965492..4965770 - 279 WP_001315112.1 hypothetical protein -
LWS57_RS23985 (4965822) 4965822..4966043 - 222 WP_001550354.1 hypothetical protein -
LWS57_RS23990 (4966128) 4966128..4966418 - 291 WP_000356395.1 NadS family protein Antitoxin
LWS57_RS23995 (4966419) 4966419..4966730 - 312 WP_000897305.1 hypothetical protein Toxin
LWS57_RS24000 (4966959) 4966959..4967867 + 909 WP_001550353.1 alpha/beta hydrolase -
LWS57_RS24005 (4968035) 4968035..4968949 - 915 WP_109553727.1 transposase -
LWS57_RS24010 (4968962) 4968962..4969849 - 888 Protein_4708 hypothetical protein -
LWS57_RS24015 (4970265) 4970265..4971206 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
LWS57_RS24020 (4971251) 4971251..4971688 - 438 WP_000560983.1 D-aminoacyl-tRNA deacylase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T294852 WP_000897305.1 NZ_OU701449:c4966730-4966419 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 10.8907

>AT294852 WP_000356395.1 NZ_OU701449:c4966418-4966128 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References