Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1211527..1212254 Replicon chromosome
Accession NZ_OU701449
Organism Escherichia coli isolate CNR85I8

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag LWS57_RS05870 Protein ID WP_000547555.1
Coordinates 1211527..1211838 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag LWS57_RS05875 Protein ID WP_000126294.1
Coordinates 1211835..1212254 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LWS57_RS05845 (1207462) 1207462..1209171 + 1710 WP_001288122.1 formate hydrogenlyase subunit HycE -
LWS57_RS05850 (1209181) 1209181..1209723 + 543 WP_000493785.1 formate hydrogenlyase subunit HycF -
LWS57_RS05855 (1209723) 1209723..1210490 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
LWS57_RS05860 (1210487) 1210487..1210897 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
LWS57_RS05865 (1210890) 1210890..1211360 + 471 WP_001551542.1 hydrogenase maturation peptidase HycI -
LWS57_RS05870 (1211527) 1211527..1211838 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
LWS57_RS05875 (1211835) 1211835..1212254 + 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
LWS57_RS05880 (1212333) 1212333..1213757 - 1425 WP_000110355.1 6-phospho-beta-glucosidase AscB -
LWS57_RS05885 (1213766) 1213766..1215223 - 1458 WP_001107889.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
LWS57_RS05890 (1215483) 1215483..1216493 + 1011 WP_029130985.1 DNA-binding transcriptional regulator AscG -
LWS57_RS05895 (1216642) 1216642..1217169 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T294837 WP_000547555.1 NZ_OU701449:1211527-1211838 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT294837 WP_000126294.1 NZ_OU701449:1211835-1212254 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References