Detailed information of TA system TA029479

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 2260352..2261271
• Replicon: chromosome
• Accession: NC_021251
• Organism: Mycobacterium tuberculosis CCDC5079

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: L7N4R2
• Locus tag: CFBS_RS21420
• Protein ID: WP_003900449.1
• Coordinates: 2260666..2261271 (-)
• Length: 202 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: L7N5K9
• Locus tag: CFBS_RS10635
• Protein ID: WP_003410124.1
• Coordinates: 2260352..2260657 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CFBS_RS10595	2255615..2255815	+	201	Protein_2072	hypothetical protein	-
CFBS_RS10610	2257171..2257548	+	378	Protein_2074	hypothetical protein	-
CFBS_RS10615	2257545..2258585	+	1041	WP_003410103.1	ImmA/IrrE family metallo-endopeptidase	-
CFBS_RS10620	2258827..2259546	+	720	WP_003410108.1	DUF433 domain-containing protein	-
CFBS_RS10625	2259536..2259952	+	417	WP_003410114.1	hypothetical protein	-
CFBS_RS10630	2259968..2260267	-	300	WP_003410120.1	hypothetical protein	-
CFBS_RS10635	2260352..2260657	-	306	WP_003410124.1	XRE family transcriptional regulator	Antitoxin
CFBS_RS21420	2260666..2261271	-	606	WP_003900449.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
CFBS_RS10645	2261296..2261655	-	360	WP_003410131.1	hypothetical protein	-
CFBS_RS10650	2261815..2262492	-	678	WP_003905725.1	hypothetical protein	-
CFBS_RS10655	2262444..2262680	-	237	WP_003410133.1	hypothetical protein	-
CFBS_RS10660	2262887..2263783	-	897	WP_003410136.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (86-181)

Antitoxin

• HigA2-like, helix-turn-helix domain (32-92)

Sequences

Toxin

Protein

• Length: 202 a.a.
• Molecular weight: 22744.96 Da
• Isoelectric Point: 7.3073

>T29479 WP_003900449.1 NC_021251:c2261271-2260666 [Mycobacterium tuberculosis CCDC5079]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Nucleotide

• Length: 606 bp

>T29479 NC_021251:c2261271-2260666 [Mycobacterium tuberculosis CCDC5079]
GTGAACGTTCCATGGGAGAACGCGCACGGCGGCGCACTGTACTGCCTCATACGTGGGGATGAATTCTCGGCCTGGCACAG
GCTACTGTTTCAACGGCCCGGGTGCGCAGAGTCCGTCCTCGCCTGCCGGCATTTCCTCGACGGTAGCCCCGTCGCCCGCT
GCTCTTACCCGGAGGAATATCACCCTTGTGTGATATCGCGTATCGCGCTACTCTGCGACTCTGTGGGGTGGACGGCGGAT
GTGGAGCGCATCTCCGCCTGGCTCAACGGCTTGGACCGAGAGACCTACGAATTGGTGTTCGCAGCTATCGAGGTTCTCGA
GGAAGAGGGCCCCGCACTTGGCTGTCCTCTGGTGGACACGGTGCGTGGGTCGCGCCACAAGAACATGAAGGAGCTGCGTC
CAGGATCACAAGGGCGGTCCGAGGTCCGCATTCTGTTCGCCTTCGACCCGGCGCGGCAGGCGATCATGCTGGCCGCCGGT
AACAAGGCCGGGCGGTGGACCCAGTGGTACGACGAGAAGATCAAAGCGGCCGATGAGATGTTTGCCGAACATCTCGCACA
GTTCGAGGACACGAAGCCAAAGAGGCGCAAGCGCAAGAAAGGGTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11291.91 Da
• Isoelectric Point: 10.7482

>AT29479 WP_003410124.1 NC_021251:c2260657-2260352 [Mycobacterium tuberculosis CCDC5079]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Nucleotide

• Length: 306 bp

>AT29479 NC_021251:c2260657-2260352 [Mycobacterium tuberculosis CCDC5079]
ATGGCGATGACACTACGGGACATGGACGCCGTACGTCCCGTTAACCGGGAGGCGGTGGACCGGCACAAGGCACGAATGCG
CGATGAAGTGCGGGCGTTCCGGCTCCGGGAGCTGCGCGCGGCTCAGTCACTCACCCAGGTCCAGGTGGCTGCGCTGGCGC
ACATCCGGCAGAGCCGGGTATCCAGCATCGAGAACGGCGACATCGGCTCCGCACAGGTCAACACACTGCGCAAATACGTG
TCCGCTCTTGGCGGAGAACTGGATATCACCGTCCGCCTGGGAGATGAGACATTCACCCTGGCATAG

Structures

Toxin

• AlphaFold DB: A0A7U4BV20

Antitoxin

• AlphaFold DB: A0A7U4BV30