Detailed information of TA system TA294751

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 25589..26012
• Replicon: plasmid 2
• Accession: NZ_OU342920
• Organism: Escherichia coli isolate UR21/11-869

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: LIX80_RS24230
• Protein ID: WP_229484968.1
• Coordinates: 25589..25708 (-)
• Length: 40 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	25796..26012 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIX80_RS24195 (20666)	20666..20893	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
LIX80_RS24200 (20987)	20987..21673	-	687	WP_000332492.1	PAS domain-containing protein	-
LIX80_RS24205 (21863)	21863..22246	-	384	WP_000124980.1	conjugal transfer relaxosome DNA-binding protein TraM	-
LIX80_RS24210 (22568)	22568..23170	+	603	WP_000243717.1	transglycosylase SLT domain-containing protein	-
LIX80_RS24215 (23465)	23465..24286	-	822	WP_001234477.1	DUF932 domain-containing protein	-
LIX80_RS24220 (24403)	24403..24690	-	288	WP_000107547.1	hypothetical protein	-
LIX80_RS24225 (24990)	24990..25296	+	307	Protein_33	hypothetical protein	-
LIX80_RS24230 (25589)	25589..25708	-	120	WP_229484968.1	Hok/Gef family protein	Toxin
LIX80_RS24235 (25656)	25656..25808	-	153	Protein_35	FlmC family protein	-
- (25796)	25796..26012	-	217	NuclAT_0	-	Antitoxin
- (25796)	25796..26012	-	217	NuclAT_0	-	Antitoxin
- (25796)	25796..26012	-	217	NuclAT_0	-	Antitoxin
- (25796)	25796..26012	-	217	NuclAT_0	-	Antitoxin
LIX80_RS24240 (25981)	25981..26743	-	763	Protein_36	plasmid SOS inhibition protein A	-
LIX80_RS24245 (26740)	26740..27147	-	408	WP_001261250.1	conjugation system SOS inhibitor PsiB	-
LIX80_RS24250 (27308)	27308..27487	+	180	WP_000618107.1	hypothetical protein	-
LIX80_RS24255 (27571)	27571..27963	-	393	WP_000272016.1	plasmid partitioning/stability family protein	-
LIX80_RS24260 (27967)	27967..28941	-	975	WP_021534625.1	plasmid segregation protein ParM	-
LIX80_RS24265 (29181)	29181..29555	-	375	WP_000752650.1	hypothetical protein	-
LIX80_RS24270 (29555)	29555..30187	-	633	WP_000312330.1	ParA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..66803	66803

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-34)

Sequences

Toxin

Protein

• Length: 40 a.a.
• Molecular weight: 4455.25 Da
• Isoelectric Point: 8.2691

>T294751 WP_229484968.1 NZ_OU342920:c25708-25589 [Escherichia coli]
VCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESSGK

Nucleotide

• Length: 120 bp

Antitoxin

Nucleotide

• Length: 217 bp

>AT294751 NZ_OU342920:c26012-25796 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTCTTTTCAGGAAAAGTGAGTGTGGTCAGCGCGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTATCCTGTCGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTCATTAACCCACGAGGCCTCTGCATGTCTAGTCCAC

Structures

Antitoxin