Detailed information of TA system TA294480

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/-
• Location: 213213..213625
• Replicon: chromosome
• Accession: NZ_LYAM01000110
• Organism: Escherichia coli strain ECOR24

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: A8F87_RS08950
• Protein ID: WP_000132601.1
• Coordinates: 213284..213625 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	213213..213289 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A8F87_RS08940 (A8F87_07565)	210076..211665	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
A8F87_RS08945 (A8F87_07570)	211662..213056	+	1395	WP_087934634.1	type I restriction-modification system specificity subunit	-
-	213213..213289	-	77	-	-	Antitoxin
A8F87_RS08950 (A8F87_07575)	213284..213625	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
A8F87_RS08955 (A8F87_07580)	213787..215166	+	1380	WP_000443951.1	5-methylcytosine-specific restriction endonuclease subunit McrB	-
A8F87_RS08960 (A8F87_07585)	215166..216213	+	1048	Protein_191	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T294480 WP_000132601.1 NZ_LYAM01000110:213284-213625 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T294480 NZ_LYAM01000110:213284-213625 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT294480 NZ_LYAM01000110:c213289-213213 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGTGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin