Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4420795..4421470 Replicon chromosome
Accession NZ_LT991954
Organism Enterobacter hormaechei subsp. steigerwaltii isolate C309

Toxin (Protein)


Gene name higB Uniprot ID A0A837FDC1
Locus tag DBR08_RS21390 Protein ID WP_015571638.1
Coordinates 4420795..4421094 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0PXC9
Locus tag DBR08_RS21395 Protein ID WP_015571639.1
Coordinates 4421105..4421470 (+) Length 122 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DBR08_RS21380 4417172..4419364 + 2193 WP_017382756.1 type I secretion system permease/ATPase -
DBR08_RS21385 4419384..4420544 + 1161 WP_085929677.1 HlyD family efflux transporter periplasmic adaptor subunit -
DBR08_RS21390 4420795..4421094 + 300 WP_015571638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
DBR08_RS21395 4421105..4421470 + 366 WP_015571639.1 HigA family addiction module antidote protein Antitoxin
DBR08_RS21400 4421497..4422678 - 1182 WP_017382758.1 PLP-dependent aminotransferase family protein -
DBR08_RS21405 4422699..4423586 - 888 WP_015571641.1 LysR family transcriptional regulator -
DBR08_RS21410 4423684..4424286 + 603 WP_017382759.1 short chain dehydrogenase -
DBR08_RS21415 4424283..4425014 - 732 WP_017382760.1 methyltransferase domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(25-72)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11624.29 Da        Isoelectric Point: 9.6776

>T293981 WP_015571638.1 NZ_LT991954:4420795-4421094 [Enterobacter hormaechei subsp. steigerwaltii]
MINHFRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Download         Length: 300 bp

Antitoxin


Download         Length: 122 a.a.        Molecular weight: 13705.73 Da        Isoelectric Point: 9.5936

>AT293981 WP_015571639.1 NZ_LT991954:4421105-4421470 [Enterobacter hormaechei subsp. steigerwaltii]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Download         Length: 366 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FDC1

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0PXC9

References