Detailed information of TA system TA029397

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 788530..789152
• Replicon: chromosome
• Accession: NC_021172
• Organism: Hyphomicrobium denitrificans 1NES1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N0BA35
• Locus tag: HYPDE_RS03625
• Protein ID: WP_015597002.1
• Coordinates: 788871..789152 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HYPDE_RS03620
• Protein ID: WP_041320806.1
• Coordinates: 788530..788820 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYPDE_RS03600	785570..786283	-	714	WP_015596995.1	FAD-dependent oxidoreductase	-
HYPDE_RS18640	786459..786693	+	235	Protein_697	DUF488 family protein	-
HYPDE_RS03605	786714..786947	+	234	WP_015596996.1	TonB-dependent receptor	-
HYPDE_RS03610	787539..787853	-	315	WP_015596998.1	hypothetical protein	-
HYPDE_RS19480	788266..788463	+	198	WP_015597000.1	hypothetical protein	-
HYPDE_RS03620	788530..788820	-	291	WP_041320806.1	HigA family addiction module antidote protein	Antitoxin
HYPDE_RS03625	788871..789152	-	282	WP_015597002.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HYPDE_RS03630	789574..790884	+	1311	WP_015597003.1	calcium-binding protein	-
HYPDE_RS18645	791305..791862	-	558	Protein_704	tyrosine-type recombinase/integrase	-
HYPDE_RS03645	792061..792864	-	804	WP_015597006.1	anti-sigma factor	-
HYPDE_RS03650	792861..793403	-	543	WP_015597007.1	sigma-70 family RNA polymerase sigma factor	-
HYPDE_RS03655	793448..793813	-	366	WP_015597008.1	c-type cytochrome	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (4-79)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10318.50 Da
• Isoelectric Point: 5.5950

>T29397 WP_015597002.1 NC_021172:c789152-788871 [Hyphomicrobium denitrificans 1NES1]
MIIDFASKATAEFFATGKTKEGWQNVSSVAKRKLDQLASTTALSELAVPPGNHLEALAGDRDGQHSIRINDQWRVCFRWT
EAGPSEVEITDYH

Nucleotide

• Length: 282 bp

>T29397 NC_021172:c789152-788871 [Hyphomicrobium denitrificans 1NES1]
ATGATAATCGATTTCGCCTCGAAAGCGACGGCTGAATTCTTCGCTACGGGAAAGACTAAGGAGGGTTGGCAAAACGTCAG
CTCGGTGGCGAAGCGCAAACTCGACCAGCTTGCGAGCACAACGGCGTTGAGCGAACTGGCTGTTCCTCCTGGCAATCATT
TGGAGGCATTGGCTGGTGACAGAGACGGCCAGCACTCGATCCGTATCAACGACCAATGGCGAGTTTGCTTCCGTTGGACG
GAAGCGGGGCCGAGCGAAGTTGAGATTACGGATTACCACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10833.63 Da
• Isoelectric Point: 10.4471

>AT29397 WP_041320806.1 NC_021172:c788820-788530 [Hyphomicrobium denitrificans 1NES1]
MKRVSTHPGEMLDEEFLKPLNMSARSLAKEIGVPANRLTELIAERRGMTADTAIRLARYFGGSAEFWMNLQSMYDLTKAV
VEKRKVYTAIVGRRAA

Nucleotide

• Length: 291 bp

>AT29397 NC_021172:c788820-788530 [Hyphomicrobium denitrificans 1NES1]
ATGAAGCGTGTTTCGACGCATCCAGGCGAAATGTTGGACGAAGAGTTCCTAAAGCCGCTCAACATGAGTGCGCGGTCGCT
TGCCAAGGAAATCGGCGTTCCCGCTAATCGGCTGACGGAGCTGATCGCAGAGCGGCGCGGCATGACGGCAGATACGGCAA
TCCGGTTGGCGCGCTATTTCGGCGGCTCGGCAGAATTCTGGATGAACCTACAATCGATGTACGACTTGACCAAGGCGGTC
GTCGAGAAGCGGAAAGTGTATACTGCAATCGTGGGGCGCAGGGCCGCGTAA

Structures

Toxin

• AlphaFold DB: N0BA35