Detailed information of TA system TA029395

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 4942658..4943313
• Replicon: chromosome
• Accession: NC_021150
• Organism: Azotobacter vinelandii CA6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: M9YJW4
• Locus tag: AVCA6_RS22315
• Protein ID: WP_012703346.1
• Coordinates: 4942969..4943313 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AVCA6_RS22310
• Protein ID: WP_012703345.1
• Coordinates: 4942658..4942972 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVCA6_RS25430	4938400..4939143	-	744	WP_012703339.1	HNH endonuclease	-
AVCA6_RS22300	4939283..4940251	+	969	WP_012703340.1	IS110 family transposase	-
AVCA6_RS22305	4940468..4941232	-	765	WP_012703341.1	class I SAM-dependent methyltransferase	-
AVCA6_RS25435	4941526..4942389	-	864	WP_012703343.1	hypothetical protein	-
AVCA6_RS22310	4942658..4942972	-	315	WP_012703345.1	helix-turn-helix domain-containing protein	Antitoxin
AVCA6_RS22315	4942969..4943313	-	345	WP_012703346.1	hypothetical protein	Toxin
AVCA6_RS22320	4943391..4944764	-	1374	WP_012703347.1	aspartate aminotransferase family protein	-
AVCA6_RS22325	4944833..4946194	-	1362	WP_012703348.1	glutamine synthetase family protein	-
AVCA6_RS22330	4946220..4946972	-	753	WP_012703349.1	gamma-glutamyl-gamma-aminobutyrate hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4939439..4940251	812

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-88)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13080.00 Da
• Isoelectric Point: 9.8648

>T29395 WP_012703346.1 NC_021150:c4943313-4942969 [Azotobacter vinelandii CA6]
MKALFIETTSFTATVGDYLTDDEYRQLQGELLKNPDAGDVMPRTGGFRKLRWADGRRGKGKRGGLRVIYYWLLGDGQFWM
FAIYDKNELENLTAEQEKLLKQAITAELKKRGAK

Nucleotide

• Length: 345 bp

>T29395 NC_021150:c4943313-4942969 [Azotobacter vinelandii CA6]
ATGAAAGCCCTCTTCATCGAAACCACGAGCTTTACCGCCACGGTCGGCGACTACCTGACCGACGACGAATATCGGCAACT
CCAGGGGGAACTGCTGAAGAACCCCGATGCCGGCGACGTCATGCCACGCACGGGCGGCTTCCGCAAGCTGCGCTGGGCCG
ATGGGCGCAGAGGGAAGGGAAAGCGTGGTGGCTTGCGCGTCATCTACTACTGGCTGCTCGGCGATGGCCAGTTCTGGATG
TTCGCCATCTACGACAAAAATGAGCTGGAGAACCTCACTGCCGAGCAGGAGAAGCTGCTCAAGCAGGCCATCACCGCAGA
GCTGAAAAAGCGAGGTGCCAAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11995.99 Da
• Isoelectric Point: 10.4600

>AT29395 WP_012703345.1 NC_021150:c4942972-4942658 [Azotobacter vinelandii CA6]
VKKRDLFAEMMQGIGEMEAHREGKITLRQYEVEERPAPEVSASEIVALRKKMHMSQQVFARRFRISTGTLRKWEQAKAKP
NAQAAVLIRLVEQYPDMAERLAAI

Nucleotide

• Length: 315 bp

>AT29395 NC_021150:c4942972-4942658 [Azotobacter vinelandii CA6]
GTGAAAAAGCGTGATTTGTTTGCCGAGATGATGCAGGGCATCGGCGAGATGGAGGCGCACCGTGAGGGCAAGATCACTCT
GCGCCAATACGAGGTCGAAGAAAGGCCGGCGCCGGAGGTGTCGGCGAGCGAGATCGTCGCCCTGCGCAAGAAAATGCACA
TGTCCCAACAGGTGTTTGCCCGCCGCTTCCGCATCAGCACGGGCACCCTGCGCAAATGGGAGCAGGCCAAGGCCAAGCCG
AACGCACAGGCTGCCGTTCTCATCAGACTGGTCGAGCAGTACCCCGACATGGCCGAAAGGCTGGCCGCGATCTGA

Structures

Toxin

• AlphaFold DB: M9YJW4