Detailed information of TA system TA029384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 401920..402511
• Replicon: chromosome
• Accession: NC_021150
• Organism: Azotobacter vinelandii CA6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: M9Y9W6
• Locus tag: AVCA6_RS01960
• Protein ID: WP_012699108.1
• Coordinates: 401920..402234 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: AVCA6_RS01965
• Protein ID: WP_012699109.1
• Coordinates: 402227..402511 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AVCA6_RS01940	397733..398095	-	363	WP_012699102.1	hypothetical protein	-
AVCA6_RS01945	399353..399748	+	396	WP_012699105.1	hypothetical protein	-
AVCA6_RS01950	400584..400826	-	243	WP_041806872.1	hypothetical protein	-
AVCA6_RS01955	401671..401901	-	231	WP_012699107.1	hypothetical protein	-
AVCA6_RS01960	401920..402234	+	315	WP_012699108.1	hypothetical protein	Toxin
AVCA6_RS01965	402227..402511	+	285	WP_012699109.1	helix-turn-helix domain-containing protein	Antitoxin
AVCA6_RS24025	402638..403294	-	657	Protein_395	Rha family transcriptional regulator	-
AVCA6_RS01980	403291..403599	-	309	WP_012699112.1	helix-turn-helix domain-containing protein	-
AVCA6_RS01985	403727..404526	-	800	Protein_397	integrase arm-type DNA-binding domain-containing protein	-
AVCA6_RS01995	405018..406253	-	1236	WP_012699114.1	methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-81)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11527.28 Da
• Isoelectric Point: 9.7621

>T29384 WP_012699108.1 NC_021150:401920-402234 [Azotobacter vinelandii CA6]
MYTVFETGVFQRYASAIWADGEREAFITWLAANPLAGDVIPGTGGLRKVRWSRAGMGKRGGTRVIYYNALADGHIWLLIA
YTKAKLDNLPVAFLNQLKEAIEHG

Nucleotide

• Length: 315 bp

>T29384 NC_021150:401920-402234 [Azotobacter vinelandii CA6]
ATGTATACCGTCTTCGAAACCGGAGTATTCCAGCGCTATGCGAGCGCTATTTGGGCCGATGGTGAGCGTGAGGCGTTCAT
CACCTGGCTGGCTGCCAATCCTCTGGCCGGCGACGTGATTCCCGGCACCGGCGGGTTGCGCAAGGTGCGCTGGTCGCGGG
CCGGGATGGGCAAGCGTGGCGGAACGCGCGTGATCTATTACAACGCGCTCGCCGATGGCCATATCTGGCTGTTGATCGCC
TATACCAAGGCCAAGCTCGACAACCTGCCGGTGGCCTTCCTGAATCAGCTGAAAGAGGCAATCGAACATGGGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10312.91 Da
• Isoelectric Point: 10.4718

>AT29384 WP_012699109.1 NC_021150:402227-402511 [Azotobacter vinelandii CA6]
MGKEMEQFQADLLESVRQMKAGRAARATKVELTPVAEARAKVGVSQAEFAELLGVSLRTLQDWEQGRRSPSGAAKTLLRV
AVKHPEALRDLQVV

Nucleotide

• Length: 285 bp

>AT29384 NC_021150:402227-402511 [Azotobacter vinelandii CA6]
ATGGGTAAGGAAATGGAGCAGTTCCAGGCCGATCTGCTGGAATCCGTCCGGCAGATGAAGGCCGGCAGGGCGGCGCGAGC
CACGAAAGTGGAACTCACGCCCGTAGCCGAGGCGCGTGCCAAAGTTGGCGTTTCGCAGGCAGAGTTCGCCGAGCTGCTGG
GGGTGAGCCTGCGAACTTTGCAGGATTGGGAGCAAGGGCGGCGATCCCCTTCCGGGGCGGCCAAAACCCTGTTGCGCGTA
GCTGTCAAACACCCCGAAGCGCTGCGCGACCTGCAAGTGGTGTGA

Structures

Toxin

• AlphaFold DB: M9Y9W6