Detailed information of TA system TA293787

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 127493..127919
• Replicon: plasmid I
• Accession: NZ_LT906556
• Organism: Escherichia coli isolate E. coli RL465

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: RL465_RS24260
• Protein ID: WP_001312861.1
• Coordinates: 127493..127651 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	127695..127919 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RL465_RS24230	122862..123551	-	690	WP_000283380.1	conjugal transfer transcriptional regulator TraJ	-
RL465_RS24235	123738..124121	-	384	WP_053320747.1	relaxosome protein TraM	-
RL465_RS24240	124442..125044	+	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
RL465_RS24245	125341..126162	-	822	WP_001234469.1	DUF945 domain-containing protein	-
RL465_RS24250	126284..126571	-	288	WP_000107535.1	hypothetical protein	-
RL465_RS24255	126596..126802	-	207	WP_000547939.1	hypothetical protein	-
RL465_RS24260	127493..127651	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	127695..127919	-	225	NuclAT_0	-	Antitoxin
-	127695..127919	-	225	NuclAT_0	-	Antitoxin
-	127695..127919	-	225	NuclAT_0	-	Antitoxin
-	127695..127919	-	225	NuclAT_0	-	Antitoxin
RL465_RS24265	127731..127919	+	189	WP_001299721.1	hypothetical protein	-
RL465_RS24270	127931..128650	-	720	WP_001276228.1	plasmid SOS inhibition protein A	-
RL465_RS24275	128647..129081	-	435	WP_000845934.1	conjugation system SOS inhibitor PsiB	-
RL465_RS24280	129136..131094	-	1959	WP_023148339.1	ParB/RepB/Spo0J family partition protein	-
RL465_RS24285	131160..131393	-	234	WP_000005990.1	DUF905 family protein	-
RL465_RS24290	131456..131995	-	540	WP_000290791.1	single-stranded DNA-binding protein	-
RL465_RS24295	132468..132716	-	249	WP_071606928.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / aph(3')-Ia / sul3 / ant(3'')-Ia / dfrA1 / sitABCD	iroB / iroC / iroD / iroE / iroN	1..157174	157174

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T293787 WP_001312861.1 NZ_LT906556:c127651-127493 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT293787 NZ_LT906556:c127919-127695 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin