Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 4564599..4565227 Replicon chromosome
Accession NZ_LT906479
Organism Serratia ficaria strain NCTC12148

Toxin (Protein)


Gene name higB Uniprot ID A0A240CB25
Locus tag CKW09_RS21370 Protein ID WP_061797174.1
Coordinates 4564599..4564889 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A8B4GQY7
Locus tag CKW09_RS21375 Protein ID WP_015379150.1
Coordinates 4564904..4565227 (+) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CKW09_RS21360 4561177..4563198 + 2022 WP_095099340.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
CKW09_RS21365 4563229..4564368 - 1140 WP_095099344.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
CKW09_RS21370 4564599..4564889 + 291 WP_061797174.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CKW09_RS21375 4564904..4565227 + 324 WP_015379150.1 HigA family addiction module antidote protein Antitoxin
CKW09_RS21380 4565220..4565732 + 513 WP_095099347.1 M48 family metallopeptidase -
CKW09_RS21385 4565785..4566765 + 981 WP_095099350.1 Gfo/Idh/MocA family oxidoreductase -
CKW09_RS21390 4566767..4567498 + 732 WP_095099353.1 glutamine amidotransferase -
CKW09_RS21395 4567883..4568854 + 972 WP_115939816.1 TerC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-94)

Antitoxin

Cro/C1-type helix-turn-helix domain

(24-71)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11708.43 Da        Isoelectric Point: 10.0891

>T293780 WP_061797174.1 NZ_LT906479:4564599-4564889 [Serratia ficaria]
MIKSFRDRYLEQFYLEGMRSRLIPGMLERQLARKLDMLAAAQQERDLHHPTGNYYKRLSGPFQGWSSLRVNMHWRLMFQW
RHDAAERIYLDPHQDT

Download         Length: 291 bp

>T293780 NZ_LT906479:4564599-4564889 [Serratia ficaria]
ATGATTAAATCTTTCCGCGATCGCTATCTGGAGCAGTTCTATCTGGAAGGCATGCGCAGCCGTCTGATTCCCGGTATGCT
CGAACGGCAGTTGGCCAGAAAGCTCGATATGTTGGCGGCCGCGCAGCAAGAGCGCGATTTGCATCACCCCACGGGCAACT
ATTACAAGCGGCTGTCGGGGCCGTTTCAGGGATGGTCAAGTCTGCGCGTTAATATGCACTGGCGGCTGATGTTCCAGTGG
CGGCATGATGCCGCTGAAAGAATTTACCTCGATCCTCACCAAGATACCTGA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12141.78 Da        Isoelectric Point: 6.2285

>AT293780 WP_015379150.1 NZ_LT906479:4564904-4565227 [Serratia ficaria]
MLETTTREPTTVGEVLQEEYLTPLGIGQEELAKVLGVTRATVNRLCNGHRRMSVAEANLFADLFETTADFWLNLQAAHDR
WEARQADAQRVRLRPIKEILGRNITHA

Download         Length: 324 bp

>AT293780 NZ_LT906479:4564904-4565227 [Serratia ficaria]
ATGTTGGAAACCACGACTCGCGAACCCACCACGGTGGGGGAAGTGCTGCAGGAAGAGTATTTAACGCCGTTGGGCATCGG
CCAGGAAGAACTGGCGAAGGTACTGGGCGTTACCCGCGCCACCGTTAACCGCCTGTGCAATGGGCATCGGCGCATGAGCG
TTGCCGAGGCCAACCTGTTTGCCGATCTGTTCGAAACGACCGCCGACTTTTGGCTGAACCTGCAGGCGGCGCACGATCGC
TGGGAGGCCAGACAGGCCGATGCGCAACGTGTGCGGCTTCGGCCAATCAAAGAAATACTGGGACGCAATATCACTCATGC
ATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A240CB25

Antitoxin

Source ID Structure
AlphaFold DB A0A8B4GQY7

References