Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2654025..2654527 Replicon chromosome
Accession NZ_LT903847
Organism Escherichia coli O127:H6 isolate EPEC1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag BQ9550_RS13700 Protein ID WP_000767829.1
Coordinates 2654273..2654527 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BQ9550_RS13695 Protein ID WP_001259255.1
Coordinates 2654025..2654276 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BQ9550_RS13665 2649203..2650270 - 1068 WP_000080126.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
BQ9550_RS13670 2650270..2651340 - 1071 WP_000108994.1 histidinol-phosphate transaminase -
BQ9550_RS13675 2651337..2652641 - 1305 WP_001339747.1 histidinol dehydrogenase -
BQ9550_RS13680 2652647..2653546 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
BQ9550_RS13690 2653692..2653742 - 51 WP_001364200.1 his operon leader peptide -
BQ9550_RS13695 2654025..2654276 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BQ9550_RS13700 2654273..2654527 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BQ9550_RS13705 2654610..2655434 + 825 WP_000754737.1 SDR family oxidoreductase -
BQ9550_RS13710 2655480..2656409 + 930 WP_000803379.1 LysR family transcriptional regulator -
BQ9550_RS13715 2656624..2656686 + 63 WP_010723108.1 membrane protein YoeI -
BQ9550_RS13720 2656676..2658034 + 1359 WP_000019194.1 putrescine/proton symporter PlaP -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T293645 WP_000767829.1 NZ_LT903847:2654273-2654527 [Escherichia coli O127:H6]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT293645 WP_001259255.1 NZ_LT903847:2654025-2654276 [Escherichia coli O127:H6]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References