Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1793904..1794631 Replicon chromosome
Accession NZ_LT903847
Organism Escherichia coli O127:H6 isolate EPEC1

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag BQ9550_RS09180 Protein ID WP_000547555.1
Coordinates 1793904..1794215 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag BQ9550_RS09185 Protein ID WP_000126295.1
Coordinates 1794212..1794631 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BQ9550_RS09155 1789839..1791548 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
BQ9550_RS09160 1791558..1792100 + 543 WP_000493801.1 formate hydrogenlyase subunit HycF -
BQ9550_RS09165 1792100..1792867 + 768 WP_000067411.1 formate hydrogenlyase subunit HycG -
BQ9550_RS09170 1792864..1793274 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
BQ9550_RS09175 1793267..1793737 + 471 WP_000132968.1 hydrogenase maturation peptidase HycI -
BQ9550_RS09180 1793904..1794215 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
BQ9550_RS09185 1794212..1794631 + 420 WP_000126295.1 helix-turn-helix domain-containing protein Antitoxin
BQ9550_RS09190 1794723..1795151 - 429 WP_000536065.1 DUF4259 domain-containing protein -
BQ9550_RS09195 1795303..1795761 - 459 WP_000526113.1 IS200/IS605-like element IS200C family transposase -
BQ9550_RS09205 1795899..1797272 - 1374 WP_000244358.1 IS4-like element ISEc13 family transposase -
BQ9550_RS09215 1797806..1798333 + 528 WP_001078777.1 electron transport protein HydN -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T293644 WP_000547555.1 NZ_LT903847:1793904-1794215 [Escherichia coli O127:H6]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15406.38 Da        Isoelectric Point: 4.4596

>AT293644 WP_000126295.1 NZ_LT903847:1794212-1794631 [Escherichia coli O127:H6]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMSV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure

References