Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3694962..3695656 Replicon chromosome
Accession NZ_LT883142
Organism Escherichia coli isolate 6666666.257727.embl

Toxin (Protein)


Gene name yafO Uniprot ID -
Locus tag EC1094V2_RS20750 Protein ID WP_096321276.1
Coordinates 3694962..3695360 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1FJN6
Locus tag EC1094V2_RS20755 Protein ID WP_000554757.1
Coordinates 3695363..3695656 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC1094V2_RS20720 3689962..3691206 - 1245 WP_000189541.1 esterase FrsA -
- 3690622..3690702 - 81 NuclAT_9 - -
- 3690622..3690702 - 81 NuclAT_9 - -
- 3690622..3690702 - 81 NuclAT_9 - -
- 3690622..3690702 - 81 NuclAT_9 - -
EC1094V2_RS20725 3691298..3691756 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
EC1094V2_RS20730 3692017..3693474 + 1458 WP_001293003.1 cytosol nonspecific dipeptidase -
EC1094V2_RS20735 3693531..3694052 - 522 Protein_3488 peptide chain release factor H -
EC1094V2_RS20740 3694051..3694254 - 204 Protein_3489 RNA ligase RtcB family protein -
EC1094V2_RS20745 3694500..3694952 - 453 WP_001059847.1 GNAT family N-acetyltransferase -
EC1094V2_RS20750 3694962..3695360 - 399 WP_096321276.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
EC1094V2_RS20755 3695363..3695656 - 294 WP_000554757.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
EC1094V2_RS20760 3695708..3696763 - 1056 WP_001226164.1 DNA polymerase IV -
EC1094V2_RS20765 3696834..3697619 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
EC1094V2_RS20770 3697591..3699303 + 1713 Protein_3495 flagellar biosynthesis protein FlhA -
EC1094V2_RS20775 3699567..3700016 - 450 WP_096321275.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15414.79 Da        Isoelectric Point: 8.9511

>T293603 WP_096321276.1 NZ_LT883142:c3695360-3694962 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDAQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.81 Da        Isoelectric Point: 5.9050

>AT293603 WP_000554757.1 NZ_LT883142:c3695656-3695363 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB S1FJN6

References