Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 556860..557587 Replicon chromosome
Accession NZ_LT883142
Organism Escherichia coli isolate 6666666.257727.embl

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag EC1094V2_RS04535 Protein ID WP_000550189.1
Coordinates 556860..557174 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EC1094V2_RS04540 Protein ID WP_000560266.1
Coordinates 557171..557587 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EC1094V2_RS04510 553018..554004 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
EC1094V2_RS04515 554083..554775 - 693 WP_000942548.1 vancomycin high temperature exclusion protein -
EC1094V2_RS04520 554852..555355 - 504 WP_001300832.1 M48 family metallopeptidase -
EC1094V2_RS04525 555440..556576 + 1137 WP_096321379.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
EC1094V2_RS04535 556860..557174 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
EC1094V2_RS04540 557171..557587 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
EC1094V2_RS04545 557632..559650 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
EC1094V2_RS04550 560076..562427 - 2352 WP_000695486.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T293589 WP_000550189.1 NZ_LT883142:556860-557174 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT293589 WP_000560266.1 NZ_LT883142:557171-557587 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References