Detailed information of TA system TA293529

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 28008..28272
• Replicon: plasmid pWI1-incI1
• Accession: NZ_LT838199
• Organism: Escherichia coli isolate WI1 isolate

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: WI2_RS25630
• Protein ID: WP_001303307.1
• Coordinates: 28008..28160 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	28215..28272 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WI2_RS25610	23337..25499	+	2163	WP_000698351.1	DotA/TraY family protein	-
WI2_RS25615	25564..26226	+	663	WP_000653333.1	plasmid IncI1-type surface exclusion protein ExcA	-
WI2_RS25620	26298..26507	-	210	WP_000062602.1	HEAT repeat domain-containing protein	-
WI2_RS26820	26850..27026	+	177	WP_001054898.1	hypothetical protein	-
WI2_RS25625	27685..27936	+	252	WP_001291967.1	hypothetical protein	-
WI2_RS25630	28008..28160	-	153	WP_001303307.1	Hok/Gef family protein	Toxin
-	28215..28272	+	58	NuclAT_0	-	Antitoxin
-	28215..28272	+	58	NuclAT_0	-	Antitoxin
-	28215..28272	+	58	NuclAT_0	-	Antitoxin
-	28215..28272	+	58	NuclAT_0	-	Antitoxin
WI2_RS25635	28452..29660	+	1209	WP_001303305.1	IncI1-type conjugal transfer protein TrbA	-
WI2_RS25640	29679..30749	+	1071	WP_085543303.1	IncI1-type conjugal transfer protein TrbB	-
WI2_RS25645	30742..33033	+	2292	WP_001289276.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..83831	83831

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T293529 WP_001303307.1 NZ_LT838199:c28160-28008 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

Antitoxin

Nucleotide

• Length: 58 bp

>AT293529 NZ_LT838199:28215-28272 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin