Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3739772..3740313 Replicon chromosome
Accession NZ_LT838196
Organism Escherichia coli isolate WI1 isolate

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag WI2_RS19365 Protein ID WP_000615983.1
Coordinates 3740035..3740313 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag WI2_RS19360 Protein ID WP_000729703.1
Coordinates 3739772..3740032 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
WI2_RS19340 3735447..3736232 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
WI2_RS19345 3736204..3737916 + 1713 Protein_3563 flagellar biosynthesis protein FlhA -
WI2_RS19350 3738140..3738637 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
WI2_RS19355 3738813..3739562 - 750 WP_000056849.1 C40 family peptidase -
WI2_RS19360 3739772..3740032 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
WI2_RS19365 3740035..3740313 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
WI2_RS19370 3740469..3741209 + 741 WP_001225679.1 murein L,D-transpeptidase -
WI2_RS19375 3741180..3741947 - 768 WP_000333380.1 class II glutamine amidotransferase -
WI2_RS19380 3742153..3742731 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3733575..3748855 15280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T293524 WP_000615983.1 NZ_LT838196:3740035-3740313 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT293524 WP_000729703.1 NZ_LT838196:3739772-3740032 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References