Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3733575..3734269 Replicon chromosome
Accession NZ_LT838196
Organism Escherichia coli isolate WI1 isolate

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag WI2_RS19325 Protein ID WP_001263489.1
Coordinates 3733575..3733973 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag WI2_RS19330 Protein ID WP_000554758.1
Coordinates 3733976..3734269 (-) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 3729163..3729243 - 81 NuclAT_11 - -
- 3729163..3729243 - 81 NuclAT_11 - -
- 3729163..3729243 - 81 NuclAT_11 - -
- 3729163..3729243 - 81 NuclAT_11 - -
WI2_RS19295 3729839..3730297 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
WI2_RS19300 3730558..3732015 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
WI2_RS19305 3732072..3732593 - 522 Protein_3556 peptide chain release factor H -
WI2_RS19310 3732589..3732795 - 207 Protein_3557 RtcB family protein -
WI2_RS19320 3733113..3733565 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
WI2_RS19325 3733575..3733973 - 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
WI2_RS19330 3733976..3734269 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
WI2_RS19335 3734321..3735376 - 1056 WP_001226164.1 DNA polymerase IV -
WI2_RS19340 3735447..3736232 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
WI2_RS19345 3736204..3737916 + 1713 Protein_3563 flagellar biosynthesis protein FlhA -
WI2_RS19350 3738140..3738637 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 3733575..3748855 15280


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T293523 WP_001263489.1 NZ_LT838196:c3733973-3733575 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp


Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT293523 WP_000554758.1 NZ_LT838196:c3734269-3733976 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A090J8B1


Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References