Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4273570..4274172 Replicon chromosome
Accession NZ_LT827011
Organism Escherichia coli O127:H6 isolate EPEC E2348/69 variety

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag BQ9544_RS22745 Protein ID WP_000897305.1
Coordinates 4273570..4273881 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag BQ9544_RS22750 Protein ID WP_000356397.1
Coordinates 4273882..4274172 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BQ9544_RS22720 4269484..4270083 + 600 WP_001298594.1 glucose-1-phosphatase -
BQ9544_RS22725 4270077..4270949 + 873 WP_000920747.1 virulence factor BrkB family protein -
BQ9544_RS22730 4270946..4271383 + 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
BQ9544_RS22735 4271428..4272369 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
BQ9544_RS22740 4272433..4273341 - 909 WP_001386513.1 alpha/beta hydrolase -
BQ9544_RS22745 4273570..4273881 + 312 WP_000897305.1 hypothetical protein Toxin
BQ9544_RS22750 4273882..4274172 + 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
BQ9544_RS22755 4274777..4274995 + 219 WP_001314326.1 ribbon-helix-helix domain-containing protein -
BQ9544_RS22760 4275219..4276148 - 930 WP_000027712.1 formate dehydrogenase accessory protein FdhE -
BQ9544_RS22765 4276145..4276780 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
BQ9544_RS22770 4276777..4277679 - 903 WP_000331385.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T293506 WP_000897305.1 NZ_LT827011:4273570-4273881 [Escherichia coli O127:H6]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT293506 WP_000356397.1 NZ_LT827011:4273882-4274172 [Escherichia coli O127:H6]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References