Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1621233..1621795 Replicon chromosome
Accession NZ_LT827011
Organism Escherichia coli O127:H6 isolate EPEC E2348/69 variety

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag BQ9544_RS08755 Protein ID WP_000605675.1
Coordinates 1621517..1621795 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag BQ9544_RS08750 Protein ID WP_000781364.1
Coordinates 1621233..1621517 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BQ9544_RS08735 1616592..1619639 + 3048 WP_012578903.1 formate dehydrogenase-N subunit alpha -
BQ9544_RS08740 1619652..1620536 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
BQ9544_RS08745 1620529..1621182 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
BQ9544_RS08750 1621233..1621517 - 285 WP_000781364.1 HigA family addiction module antidote protein Antitoxin
BQ9544_RS08755 1621517..1621795 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BQ9544_RS08760 1621981..1622991 - 1011 WP_000642413.1 alcohol dehydrogenase AdhP -
BQ9544_RS08765 1623125..1624822 - 1698 WP_000433462.1 malate dehydrogenase -
BQ9544_RS08770 1624978..1625115 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
BQ9544_RS08775 1625217..1625432 - 216 WP_000495766.1 biofilm-dependent modulation protein -
BQ9544_RS08785 1625777..1626208 + 432 WP_000152307.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(17-65)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T293494 WP_000605675.1 NZ_LT827011:c1621795-1621517 [Escherichia coli O127:H6]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10386.11 Da        Isoelectric Point: 8.8567

>AT293494 WP_000781364.1 NZ_LT827011:c1621517-1621233 [Escherichia coli O127:H6]
MKMANHPRPGDIILESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure

References