Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pmenTA/darT(toxin)
Location 1154098..1155818 Replicon chromosome
Accession NZ_LT827011
Organism Escherichia coli O127:H6 isolate EPEC E2348/69 variety

Toxin (Protein)


Gene name pmenT Uniprot ID B7UP20
Locus tag BQ9544_RS06060 Protein ID WP_000312835.1
Coordinates 1155162..1155818 (-) Length 219 a.a.

Antitoxin (Protein)


Gene name pmenA Uniprot ID B7UP19
Locus tag BQ9544_RS06055 Protein ID WP_000634203.1
Coordinates 1154098..1155165 (-) Length 356 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BQ9544_RS06040 1149898..1150098 - 201 Protein_1111 integrase core domain-containing protein -
BQ9544_RS06045 1150268..1150984 - 717 WP_001122107.1 M48 family metallopeptidase -
BQ9544_RS06050 1150984..1154079 - 3096 WP_000108733.1 HsdR family type I site-specific deoxyribonuclease -
BQ9544_RS06055 1154098..1155165 - 1068 WP_000634203.1 macro domain-containing protein Antitoxin
BQ9544_RS06060 1155162..1155818 - 657 WP_000312835.1 DUF4433 domain-containing protein Toxin
BQ9544_RS06065 1155830..1157179 - 1350 WP_001386438.1 restriction endonuclease subunit S -
BQ9544_RS06070 1157191..1159629 - 2439 WP_000985463.1 type I restriction-modification system subunit M -
BQ9544_RS06075 1159669..1159908 - 240 WP_000343040.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - nleE / nleB1 / nleB1 / espL2 / espL2 1117747..1177570 59823
inside Prophage - nleE / nleB1 / nleB1 / espL2 / espL2 1101582..1177570 75988


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

ssDNA thymidine ADP-ribosyltransferase, DarT

(17-217)

Antitoxin

Macro domain

(18-129)


Sequences

Toxin        


Download         Length: 219 a.a.        Molecular weight: 25276.63 Da        Isoelectric Point: 6.5796

>T293491 WP_000312835.1 NZ_LT827011:c1155818-1155162 [Escherichia coli O127:H6]
MAYDYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSLVQAENWINIGNPELIGKRAGHPVPVGTGGTLHDYVPFYFTP
FSPMLMNIHSGRGGIKRRPNEEIVILVSNLRNVAAHDVPFVFTDSHAYYNWTNYYTSLNSLDQIDWPILQARDFRRDPDD
PAKFERYQAEALIWQHCPISLLDGIICYSEEVRLQLEQWLFQRNLTMSVHTRSGWYFS

Download         Length: 657 bp

Antitoxin


Download         Length: 356 a.a.        Molecular weight: 40672.70 Da        Isoelectric Point: 5.6927

>AT293491 WP_000634203.1 NZ_LT827011:c1155165-1154098 [Escherichia coli O127:H6]
MITYTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPENMKVYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRADSRMEWIEDGLQDLRRFLIEENVQSIAIPPLGAGNGGLNWPDVRAQIESALGDLQDVDILIYQPTEKYQNVAKST
GVKKLTPARAAIAELVRRYWVLGMECSLLEIQKLAWLLQRAIEQHQQDDILKLRFEAHYYGPYAPNLNHLLNALDGTYLK
AEKRIPDSQPLDVIWFNDQKKEHVNAYLNNEAREWLPALEQVSQLIDGFESPFGLELLATVDWLLSRGECQPTLDSVKEG
LHQWPAGERWASRKLRLFDNNNLQFAINRVMEFHC

Download         Length: 1068 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A376HLR4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9J2B9

References