Detailed information of TA system TA293480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4792636..4793238
• Replicon: chromosome
• Accession: NZ_LT795502
• Organism: Escherichia coli strain KV7 isolate KV7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: KJB12_RS22705
• Protein ID: WP_000897305.1
• Coordinates: 4792927..4793238 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KJB12_RS22700
• Protein ID: WP_000356397.1
• Coordinates: 4792636..4792926 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJB12_RS22675 (4788583)	4788583..4789485	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
KJB12_RS22680 (4789482)	4789482..4790117	+	636	WP_000829031.1	formate dehydrogenase cytochrome b556 subunit	-
KJB12_RS22685 (4790114)	4790114..4791043	+	930	WP_000027691.1	formate dehydrogenase accessory protein FdhE	-
KJB12_RS22690 (4791373)	4791373..4791615	-	243	WP_001086385.1	protein YiiF	-
KJB12_RS22695 (4791834)	4791834..4792052	-	219	WP_001313440.1	CopG family transcriptional regulator	-
KJB12_RS22700 (4792636)	4792636..4792926	-	291	WP_000356397.1	NadS family protein	Antitoxin
KJB12_RS22705 (4792927)	4792927..4793238	-	312	WP_000897305.1	hypothetical protein	Toxin
KJB12_RS22710 (4793467)	4793467..4794375	+	909	WP_023155176.1	alpha/beta hydrolase	-
KJB12_RS26955 (4794543)	4794543..4795457	-	915	WP_191663692.1	transposase	-
KJB12_RS26960 (4795467)	4795467..4796357	-	891	Protein_4468	hypothetical protein	-
KJB12_RS22720 (4796768)	4796768..4797709	-	942	WP_001343389.1	fatty acid biosynthesis protein FabY	-
KJB12_RS22725 (4797754)	4797754..4798191	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12172.16 Da
• Isoelectric Point: 9.7791

>T293480 WP_000897305.1 NZ_LT795502:c4793238-4792927 [Escherichia coli]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T293480 NZ_LT795502:c4793238-4792927 [Escherichia coli]
ATACTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT293480 WP_000356397.1 NZ_LT795502:c4792926-4792636 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT293480 NZ_LT795502:c4792926-4792636 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7