Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 445558..446148 Replicon chromosome
Accession NZ_LT605586
Organism Brucella inopinata strain 141012304 isolate Brucella sp.

Toxin (Protein)


Gene name graT Uniprot ID A0A7L7MSL3
Locus tag BR141012304_RS12305 Protein ID WP_069714895.1
Coordinates 445867..446148 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID A0A7L7MR57
Locus tag BR141012304_RS12300 Protein ID WP_008510700.1
Coordinates 445558..445854 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BR141012304_RS12265 441102..441809 - 708 WP_083331527.1 3'-5' exoribonuclease -
BR141012304_RS12270 441878..442066 - 189 Protein_395 hypothetical protein -
BR141012304_RS12275 442213..442401 - 189 WP_008510705.1 hypothetical protein -
BR141012304_RS12280 442547..442852 - 306 WP_002971236.1 hypothetical protein -
BR141012304_RS12285 442993..443370 + 378 WP_070998261.1 pyrimidine dimer DNA glycosylase/endonuclease V -
BR141012304_RS12290 444274..444993 + 720 WP_070998263.1 GGDEF domain-containing protein -
BR141012304_RS12300 445558..445854 - 297 WP_008510700.1 HigA family addiction module antidote protein Antitoxin
BR141012304_RS12305 445867..446148 - 282 WP_069714895.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BR141012304_RS12315 446906..449095 - 2190 WP_070998266.1 esterase-like activity of phytase family protein -
BR141012304_RS12320 449267..450607 - 1341 WP_070998268.1 DNA polymerase IV -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 431218..464022 32804


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(16-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(17-68)


Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10569.00 Da        Isoelectric Point: 8.0374

>T293291 WP_069714895.1 NZ_LT605586:c446148-445867 [Brucella inopinata]
MIRSYKNSVTETVAWGKAPKGFPSDLVRVSVRKLTMLDNAVTLDDLRSPPGNRLEALKGDRQGQHSIRINDQWRICFVWT
DAGAEQVEIVDYH

Download         Length: 282 bp

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11001.76 Da        Isoelectric Point: 5.6882

>AT293291 WP_008510700.1 NZ_LT605586:c445854-445558 [Brucella inopinata]
MTNILPPVHPGEILREEYLIPLGMSAGALAKRLNVPRTRIERLATEQTNVTPDTALRLAKFLRTTPEFWMNMQASYDLKT
EAVAIQDQLAAIHEMEAA

Download         Length: 297 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7L7MSL3

Antitoxin

Source ID Structure
AlphaFold DB A0A7L7MR57

References