Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2977977..2978518 Replicon chromosome
Accession NZ_LT601384
Organism Escherichia coli isolate NCTC86EC

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag NCTC86EC_RS14670 Protein ID WP_000615983.1
Coordinates 2978240..2978518 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag NCTC86EC_RS14665 Protein ID WP_000729703.1
Coordinates 2977977..2978237 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NCTC86EC_RS14645 2973652..2974437 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
NCTC86EC_RS14650 2974409..2976121 + 1713 Protein_2804 flagellar biosynthesis protein FlhA -
NCTC86EC_RS14655 2976345..2976842 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
NCTC86EC_RS14660 2977018..2977767 - 750 WP_000056849.1 C40 family peptidase -
NCTC86EC_RS14665 2977977..2978237 + 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
NCTC86EC_RS14670 2978240..2978518 + 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
NCTC86EC_RS14675 2978674..2979414 + 741 WP_001225679.1 murein L,D-transpeptidase -
NCTC86EC_RS14680 2979385..2980152 - 768 WP_000333380.1 class II glutamine amidotransferase -
NCTC86EC_RS14685 2980358..2980936 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 2971780..2987061 15281


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T293273 WP_000615983.1 NZ_LT601384:2978240-2978518 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT293273 WP_000729703.1 NZ_LT601384:2977977-2978237 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References