Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 2971780..2972474 Replicon chromosome
Accession NZ_LT601384
Organism Escherichia coli isolate NCTC86EC

Toxin (Protein)


Gene name yafO Uniprot ID A0A3P5GWS5
Locus tag NCTC86EC_RS14630 Protein ID WP_001263488.1
Coordinates 2971780..2972178 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag NCTC86EC_RS14635 Protein ID WP_000554758.1
Coordinates 2972181..2972474 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
- 2967368..2967448 - 81 NuclAT_12 - -
- 2967368..2967448 - 81 NuclAT_12 - -
- 2967368..2967448 - 81 NuclAT_12 - -
- 2967368..2967448 - 81 NuclAT_12 - -
NCTC86EC_RS14605 2968044..2968502 - 459 WP_001291990.1 xanthine phosphoribosyltransferase -
NCTC86EC_RS14610 2968763..2970220 + 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
NCTC86EC_RS14615 2970277..2970798 - 522 Protein_2797 peptide chain release factor H -
NCTC86EC_RS14620 2970794..2971000 - 207 Protein_2798 RtcB family protein -
NCTC86EC_RS14625 2971318..2971770 - 453 WP_001059892.1 GNAT family N-acetyltransferase -
NCTC86EC_RS14630 2971780..2972178 - 399 WP_001263488.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
NCTC86EC_RS14635 2972181..2972474 - 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
NCTC86EC_RS14640 2972526..2973581 - 1056 WP_001226164.1 DNA polymerase IV -
NCTC86EC_RS14645 2973652..2974437 - 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
NCTC86EC_RS14650 2974409..2976121 + 1713 Protein_2804 flagellar biosynthesis protein FlhA -
NCTC86EC_RS14655 2976345..2976842 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 2971780..2987061 15281


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15559.90 Da        Isoelectric Point: 6.9798

>T293272 WP_001263488.1 NZ_LT601384:c2972178-2971780 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQEAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT293272 WP_000554758.1 NZ_LT601384:c2972474-2972181 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3P5GWS5

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References