Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4229189..4229988 Replicon chromosome
Accession NZ_LT594504
Organism Escherichia coli isolate E. coli RL465

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag RL465_RS20405 Protein ID WP_000347251.1
Coordinates 4229524..4229988 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag RL465_RS20400 Protein ID WP_001307405.1
Coordinates 4229189..4229524 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RL465_RS20385 4224974..4225744 - 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -
RL465_RS20390 4225760..4227094 - 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
RL465_RS20395 4227469..4229040 + 1572 WP_001273761.1 galactarate dehydratase -
RL465_RS20400 4229189..4229524 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
RL465_RS20405 4229524..4229988 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
RL465_RS20410 4230043..4230852 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
RL465_RS20415 4231101..4232381 + 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
RL465_RS20420 4232404..4232877 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
RL465_RS20425 4232888..4233667 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
RL465_RS20430 4233657..4234535 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
RL465_RS20435 4234553..4234987 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4218314..4229988 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T293227 WP_000347251.1 NZ_LT594504:4229524-4229988 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT293227 WP_001307405.1 NZ_LT594504:4229189-4229524 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References