Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3719884..3720611 Replicon chromosome
Accession NZ_LT594504
Organism Escherichia coli isolate E. coli RL465

Toxin (Protein)


Gene name higB Uniprot ID J7Q991
Locus tag RL465_RS17945 Protein ID WP_000547564.1
Coordinates 3720300..3720611 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag RL465_RS17940 Protein ID WP_000126294.1
Coordinates 3719884..3720303 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
RL465_RS17920 3714934..3715461 - 528 WP_001078777.1 electron transport protein HydN -
RL465_RS17925 3715610..3716620 - 1011 WP_001392554.1 DNA-binding transcriptional regulator AscG -
RL465_RS17930 3716880..3718337 + 1458 WP_001107861.1 PTS cellobiose/arbutin/salicin transporter subunit IIBC -
RL465_RS17935 3718346..3719770 + 1425 WP_000110363.1 6-phospho-beta-glucosidase AscB -
RL465_RS17940 3719884..3720303 - 420 WP_000126294.1 helix-turn-helix domain-containing protein Antitoxin
RL465_RS17945 3720300..3720611 - 312 WP_000547564.1 type II toxin-antitoxin system HigB family toxin Toxin
RL465_RS17950 3720785..3721546 - 762 WP_001026446.1 hypothetical protein -
RL465_RS17955 3721571..3722041 - 471 WP_000132961.1 hydrogenase maturation peptidase HycI -
RL465_RS17960 3722034..3722444 - 411 WP_001291921.1 formate hydrogenlyase assembly protein HycH -
RL465_RS17965 3722441..3723208 - 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
RL465_RS17970 3723208..3723750 - 543 WP_000493792.1 formate hydrogenlyase subunit HycF -
RL465_RS17975 3723760..3725469 - 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12426.13 Da        Isoelectric Point: 9.7248

>T293222 WP_000547564.1 NZ_LT594504:c3720611-3720300 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15416.42 Da        Isoelectric Point: 4.4596

>AT293222 WP_000126294.1 NZ_LT594504:c3720303-3719884 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Download         Length: 420 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FJG5

Antitoxin

Source ID Structure

References