Detailed information of TA system TA293088

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 59495..59759
• Replicon: plasmid 3
• Accession: NZ_LS999562
• Organism: Escherichia coli isolate EC-TO143

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: ECTO143_RS26225
• Protein ID: WP_001331364.1
• Coordinates: 59607..59759 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	59495..59552 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECTO143_RS26210	54734..57025	-	2292	WP_001289276.1	hypothetical protein	-
ECTO143_RS26215	57018..58088	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
ECTO143_RS26220	58107..59315	-	1209	WP_016245570.1	IncI1-type conjugal transfer protein TrbA	-
-	59495..59552	-	58	NuclAT_1	-	Antitoxin
-	59495..59552	-	58	NuclAT_1	-	Antitoxin
-	59495..59552	-	58	NuclAT_1	-	Antitoxin
-	59495..59552	-	58	NuclAT_1	-	Antitoxin
ECTO143_RS26225	59607..59759	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
ECTO143_RS26230	59831..60082	-	252	WP_032329894.1	hypothetical protein	-
ECTO143_RS27080	60741..60917	-	177	WP_001054897.1	hypothetical protein	-
ECTO143_RS26235	61309..61518	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
ECTO143_RS26240	61590..62240	-	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
ECTO143_RS26245	62314..64415	-	2102	Protein_74	DotA/TraY family protein	-
ECTO143_RS26250	64518..64721	-	204	Protein_75	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-1	-	1..102557	102557

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T293088 WP_001331364.1 NZ_LS999562:59607-59759 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

Antitoxin

Nucleotide

• Length: 58 bp

>AT293088 NZ_LS999562:c59552-59495 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin