Detailed information of TA system TA293084

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 85964..86390
• Replicon: plasmid 2
• Accession: NZ_LS999561
• Organism: Escherichia coli isolate EC-TO143

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: ECTO143_RS25455
• Protein ID: WP_001312861.1
• Coordinates: 85964..86122 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	86166..86390 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECTO143_RS25405	81021..81718	+	698	Protein_90	IS1 family transposase	-
ECTO143_RS25410	81653..82009	-	357	WP_134089488.1	PAS domain-containing protein	-
ECTO143_RS25415	82203..82586	-	384	WP_001151564.1	relaxosome protein TraM	-
ECTO143_RS25420	82872..83519	+	648	WP_000614282.1	transglycosylase SLT domain-containing protein	-
ECTO143_RS25425	83816..84637	-	822	WP_001234469.1	DUF945 domain-containing protein	-
ECTO143_RS25430	84758..85045	-	288	WP_000107537.1	hypothetical protein	-
ECTO143_RS25455	85964..86122	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	86166..86390	-	225	NuclAT_0	-	Antitoxin
-	86166..86390	-	225	NuclAT_0	-	Antitoxin
-	86166..86390	-	225	NuclAT_0	-	Antitoxin
-	86166..86390	-	225	NuclAT_0	-	Antitoxin
ECTO143_RS27070	86202..86390	+	189	WP_001299721.1	hypothetical protein	-
ECTO143_RS25460	86402..87121	-	720	WP_001276228.1	plasmid SOS inhibition protein A	-
ECTO143_RS25465	87118..87552	-	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
ECTO143_RS25470	87607..89565	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
ECTO143_RS25475	89624..89857	-	234	WP_000006004.1	DUF905 domain-containing protein	-
ECTO143_RS25480	89913..90440	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
ECTO143_RS25505	90910..91158	-	249	WP_071606928.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sitABCD	-	1..134692	134692

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T293084 WP_001312861.1 NZ_LS999561:c86122-85964 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

Antitoxin

Nucleotide

• Length: 225 bp

>AT293084 NZ_LS999561:c86390-86166 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin